Pischedda C, Cocco S, Melis A, Marini M G, Kan Y W, Cao A, Moi P
Istituto di Clinica e Biologia dell' Età Evolutiva, Università di Cagliari, Italy.
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3511-5. doi: 10.1073/pnas.92.8.3511.
The transcription factor NF-E2 (nuclear factor erythroid 2), interacting via DNA motifs within regulatory regions of several hematopoietic genes, is thought to mediate the enhancer activity of the globin locus control regions. By screening a human fetal liver cDNA library with probes derived from mouse NF-E2, we have isolated a splicing variant of the NF-E2 gene (fNF-E2) that differs in the 5' untranslated region from the previously reported cDNA (aNF-E2). The fNF-E2 isoform is transcribed from an alternative promoter located in the 3' end of the first intron and joined by alternative splicing to the second and third exons, which are shared by both RNA isoforms. Although the two forms produce the same protein, they are expressed in different ratios during development. fNF-E2 is more abundant in the fetal liver and less abundant in the adult bone marrow compared to the previously described form. Their distribution apparently follows the differential expression of fetal and adult hemoglobins.
转录因子NF-E2（核因子红细胞2）通过与多个造血基因调控区域内的DNA基序相互作用，被认为介导了珠蛋白基因座控制区域的增强子活性。通过用源自小鼠NF-E2的探针筛选人胎儿肝脏cDNA文库，我们分离出了NF-E2基因的一个剪接变体（fNF-E2），其5'非翻译区与先前报道的cDNA（aNF-E2）不同。fNF-E2同工型从位于第一个内含子3'端的一个替代启动子转录而来，并通过替代剪接与RNA同工型共有的第二和第三个外显子相连。虽然这两种形式产生相同的蛋白质，但它们在发育过程中以不同的比例表达。与先前描述的形式相比，fNF-E2在胎儿肝脏中更为丰富，而在成人骨髓中则较少。它们的分布显然与胎儿和成人血红蛋白的差异表达一致。
