Chan J Y, Han X L, Kan Y W
Department of Laboratory Medicine, University of California, San Francisco 94143-0724.
Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11366-70. doi: 10.1073/pnas.90.23.11366.
The human homolog of mouse NF-E2 was isolated from the K562 cell line and found to encode a member of the basic leucine-zipper family of DNA-binding regulatory proteins. The deduced amino acid sequence of the mouse and human proteins exhibited near identity. Comparison to the related protein, Nrf1, revealed significant homologies at isolated regions, particularly within the basic domain, suggesting that NF-E2 and Nrf1 are members of a distinct subfamily of basic leucine-zipper proteins that share similar DNA-binding properties. High levels of human NF-E2 mRNA were observed in human erythroleukemic cell lines examined. Extensive survey of human tissue samples found NF-E2 expression not limited to erythropoeitic organs. Expression in the colon and testis suggests that NF-E2 may participate in the regulation of genes other than globin.
从小鼠NF-E2的人同源物是从K562细胞系中分离得到的，发现它编码一种DNA结合调节蛋白的碱性亮氨酸拉链家族成员。推导的小鼠和人蛋白质的氨基酸序列显示出近乎相同。与相关蛋白Nrf1比较，发现在分离区域有显著同源性，特别是在碱性结构域内，这表明NF-E2和Nrf1是具有相似DNA结合特性的碱性亮氨酸拉链蛋白独特亚家族的成员。在所检测的人红白血病细胞系中观察到高水平的人NF-E2 mRNA。对人体组织样本的广泛调查发现NF-E2的表达不限于造血器官。在结肠和睾丸中的表达表明NF-E2可能参与除珠蛋白以外的基因的调控。
