Yan Z, Yan X, Luan F, Yan X
Speciality Institute of CAAS, Jilin.
Wei Sheng Wu Xue Bao. 1995 Feb;35(1):28-32.
It is the first time that this paper reports on using improved rabbit blood agar culture medium to isolate 145 strains Gardnerella vaginalis from the foxes and their abortion fortus organs of main fox farms. Among the strains, 26 strains were isolated from the abortion foetus organs, isolation rate was 92.86%; 118 strains were isolated from the vaginal exeretion of the abortion and empty foxes, isolation rate was 34.01%; 1 strain was isolated from inguinal lymph node of the pelted positive fox, isolation rate was 2%. None was isolated from blood. By causative agent isolation, we revealed the bacterium survival position in vivo, and the best isolation route isolation opportunity and isolation methods were selected. By artificial infection test, we have proved pathogenicity of the bacterium.
本文首次报道了使用改良兔血琼脂培养基从主要狐狸养殖场的狐狸及其流产胎儿器官中分离出145株阴道加德纳菌。其中,从流产胎儿器官中分离出26株,分离率为92.86%;从流产和空怀狐狸的阴道分泌物中分离出118株,分离率为34.01%;从脱毛阳性狐狸的腹股沟淋巴结中分离出1株,分离率为2%。血液中未分离出该菌。通过病原体分离,揭示了该菌在体内的生存部位,选择了最佳的分离途径、分离时机和分离方法。通过人工感染试验,证实了该菌的致病性。