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[一种新的人畜共患病——狐阴道加德纳菌病的调查。V. 狐阴道加德纳菌血清型研究]

[A new zoonosis--investigation of Gardnerella vaginalis disease of fox. V. Studies serotype of Gardnerella vaginalis in fox].

作者信息

Yan X, Yan Z, Yan X, Luan F, Wang C

机构信息

Speciality Institute of CAAS, Jilin.

出版信息

Wei Sheng Wu Xue Bao. 1996 Oct;36(5):373-8.

PMID:9639824
Abstract

145 strains Gardnerella vaginalis isolated in foxes were isolated from 13 main farms raising foxes in six provinces (regions), China, after antigenicity and immunogenicity of the strains were measured, 1-3 appropriate strains were selected from each farm raising foxes for serotype studies. Cross agglutinin absorption test confirmed that selected 26 strains Gardnerella vaginalis were divided into three serotypes and then the representing strains were used to produce typing serum. Among remaining 119 strain, 108 strains were typable with the typing sera, and 11 strains can't be set. Among three serotypes, serotype I made up 79.1% of the strains. It was shown that serotype I was the principal serotype of Gardnerella vaginalis of fox in China. The test also confirmed that 5 strains of Gardnerella vaginalis isolated from racoon dog, 4 strain Gardnerella vaginalis from mink and 2 strains Gardnerella vaginalis from canine also belonged to serotype I. Supersonic antigben was produced with three serotypes, representative strains. By agar immuno-diffusion test, it confirmed that the antigens of three serotypes formed a obvious blending precipitating line with the homologous or heterologous serotype antiserum. It indicated common antigen existed among all serotypes. The agar immuno-diffusion test results revealed that the precipitating line of the homologous serotype completely blended. It is our opinion that the method of serotyping is reliable.

摘要

从中国六个省(区)的13个主要养狐场分离出145株阴道加德纳菌,测定这些菌株的抗原性和免疫原性后,从每个养狐场选择1 - 3株合适的菌株进行血清型研究。交叉凝集素吸收试验证实,所选的26株阴道加德纳菌分为三个血清型,然后用代表菌株制备分型血清。在其余119株中,108株可用分型血清分型,11株无法分型。在三个血清型中,血清型I占菌株的79.1%。结果表明,血清型I是中国狐源阴道加德纳菌的主要血清型。试验还证实,从貉分离的5株阴道加德纳菌、从水貂分离的4株阴道加德纳菌和从犬分离的2株阴道加德纳菌也属于血清型I。用三个血清型的代表菌株制备了超声抗原。通过琼脂免疫扩散试验证实,三个血清型的抗原与同源或异源血清型抗血清形成明显的融合沉淀线。这表明所有血清型之间存在共同抗原。琼脂免疫扩散试验结果显示同源血清型的沉淀线完全融合。我们认为血清分型方法是可靠的。

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