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[一种新的人畜共患病——狐阴道加德纳菌病的调查。IV. 斑点酶联免疫吸附测定法检测狐阴道加德纳菌病血清抗体的研究]

[A new zoonosis--investigation of Gardnerella vaginalis disease of fox. IV. Study of dot-ELISA for the detection of serum antibody of Gardnerella vaginalis disease of fox].

作者信息

Yan X, Yan Z, Yan X, Luan F, Wang C

机构信息

Speciality Institute of CAAS, Jilin.

出版信息

Wei Sheng Wu Xue Bao. 1995 Aug;35(4):287-91.

PMID:7483585
Abstract

192 Gardnerella vaginalis antibody positive sera from artifically infected foxes, immunized foxes and foxes that the bacterial examination were positive were analysed by the established Dot-ELISA. The results showed that 187/192 were positive, the positive rate was 97.3%. Block and cross tests showed that the method was specific. The method was 28.1 times sensitive as PAT and 5.5 times sensitive as MAT respectively. The repeatability of the test was good and the positive coincidence rate among the three techniques was 100%.

摘要

用建立的斑点酶联免疫吸附测定法(Dot-ELISA)分析了192份来自人工感染狐狸、免疫狐狸以及细菌检查呈阳性的狐狸的阴道加德纳菌抗体阳性血清。结果显示,192份血清中有187份呈阳性,阳性率为97.3%。阻断试验和交叉试验表明该方法具有特异性。该方法的灵敏度分别是试管凝集试验(PAT)的28.1倍和微量凝集试验(MAT)的5.5倍。该检测方法重复性良好,三种技术之间的阳性符合率为100%。

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