Mallet L, Bussereau F, Jacquet M
Isntitut de Genétique et Microbiologie, URA1354 du CNRS, Laboratoire Information Genétique et Développement, Université Paris-Sud, Orsay, France.
Yeast. 1995 Sep 30;11(12):1195-209. doi: 10.1002/yea.320111210.
A 43,481 bp fragment from the left arm of chromosome XIV of Saccharomyces cerevisiae was sequenced. A gene for tRNA(phe) and 23 non-overlapping open reading frames (ORFs) were identified, seven of which correspond to known yeast genes: MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1. One ORF may correspond to the yet unidentified yeast adenosine deaminase gene. Among the 15 other ORFs, four exhibit known signatures, which include a protein tyrosine phosphatase, a cytoskeleton-associated protein and two ATP-binding proteins, four have similarities with putative proteins of yeast or proteins from other organisms and seven exibit no significant similarity with amino acid sequences described in data banks. One ORF is identical to yeast expressed sequence tags (EST) and therefore corresponds to an expressed gene. Six ORFs present similarities to human dbESTs, thus identifying motifs conserved during evolution. Nine ORFs are putative transmembrane proteins. In addition, one overlapping and three antisense ORFs, which are not likely to be functional, were detected.
对酿酒酵母第十四条染色体左臂上一段43481 bp的片段进行了测序。鉴定出一个tRNA（苯丙氨酸）基因和23个不重叠的开放阅读框（ORF），其中7个与已知的酵母基因相对应：MFA2、MEP2、CAP/SRV2、NAM9、FKB1/FPR1/RBP1、MOM22和CPT1。一个ORF可能对应尚未鉴定的酵母腺苷脱氨酶基因。在其他15个ORF中，4个具有已知特征，包括一种蛋白质酪氨酸磷酸酶、一种细胞骨架相关蛋白和两种ATP结合蛋白，4个与酵母的推定蛋白或其他生物的蛋白具有相似性，7个与数据库中描述的氨基酸序列无显著相似性。一个ORF与酵母表达序列标签（EST）相同，因此对应一个已表达基因。6个ORF与人类dbEST具有相似性，从而鉴定出在进化过程中保守的基序。9个ORF是推定的跨膜蛋白。此外，还检测到一个重叠ORF和三个反义ORF，它们可能没有功能。
