Alvarez R, Honrubia M P, Herráez M P
Departamento de Biologia Celular y Anatomia, Universidad de León, Spain.
Arch Environ Contam Toxicol. 1995 Apr;28(3):349-56. doi: 10.1007/BF00213113.
Tadpoles of Rana perezi were kept for 14 weeks in water containing two sublethal levels of the carbamate insecticide ZZ-Aphox or the organophosphate Folidol. Approximate concentrations of their active ingredients were 0.25 and 1 mg/L. Resulting malformations were studied by skeletal analysis and histological and histochemical investigation of te rear limbs of the tadpoles. The pesticides caused the animals to have malformations of the spinal column (scoliosis) and/or limbs (short and thick long bones with the epiphyses grossly twisted). Histochemical study showed differences in the composition of the connective matrix, and microscopic examination of the long bones indicated alterations in the thickness of the uncalcified bone matrix (osteoid) and the presence of abundant vascularised connective tissue in the region of the periosteum. The results confirmed changes in the composition of the connective tissue matrix as the cause of the defects observed in bone formation which are also discussed in relation to vitamin D absorption and calcium homeostasis.
将泽陆蛙蝌蚪置于含有两种亚致死剂量的氨基甲酸酯类杀虫剂ZZ - Aphox或有机磷杀虫剂伏杀硫磷的水中饲养14周。其活性成分的近似浓度分别为0.25毫克/升和1毫克/升。通过骨骼分析以及对蝌蚪后肢的组织学和组织化学研究，对所产生的畸形进行了研究。这些杀虫剂致使动物出现脊柱畸形（脊柱侧凸）和/或肢体畸形（长骨短粗且骨骺严重扭曲）。组织化学研究显示结缔组织基质的组成存在差异，对长骨的显微镜检查表明未钙化骨基质（类骨质）的厚度发生改变，并且在骨膜区域存在大量血管化的结缔组织。结果证实结缔组织基质的组成变化是骨形成中观察到的缺陷的原因，还结合维生素D吸收和钙稳态对这些变化进行了讨论。
