Fardeau M L, Ollivier B, Patel B K, Dwivedi P, Ragot M, Garcia J L
Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, Marseille, France.
Int J Syst Bacteriol. 1995 Apr;45(2):218-21. doi: 10.1099/00207713-45-2-218.
Strain MLFT (T = type strain), a new thermophilic, spore-forming sulfate-reducing bacterium, was characterized and was found to be phenotypically, genotypically, and phylogenetically related to the genus Desulfotomaculum. This organism was isolated from a butyrate enrichment culture that had been inoculated with a mixed compost containing rice hulls and peanut shells. The optimum temperature for growth was 50 degrees C. The G+C content of the DNA was 51.2 mol%. Strain MLFT incompletely oxidized pyruvate, butyrate, and butanol to acetate and presumably CO2. It used long-chain fatty acids and propanediols. We observed phenotypic and phylogenetic differences between strain MLFT and other thermophilic Desulfotomaculum species that also oxidize long-chain fatty acids. On the basis of our results, we propose that strain MLFT is a member of a new species, Desulfotomaculum thermosapovorans.
菌株MLFT（T = 模式菌株）是一种新的嗜热、产芽孢的硫酸盐还原菌，经鉴定发现其在表型、基因型和系统发育方面与脱硫肠状菌属相关。该菌株是从接种了含有稻壳和花生壳的混合堆肥的丁酸盐富集培养物中分离得到的。其生长的最适温度为50℃。DNA的G+C含量为51.2 mol%。菌株MLFT将丙酮酸、丁酸盐和丁醇不完全氧化为乙酸盐并推测生成二氧化碳。它利用长链脂肪酸和丙二醇。我们观察到菌株MLFT与其他也氧化长链脂肪酸的嗜热脱硫肠状菌属物种在表型和系统发育上存在差异。基于我们的研究结果，我们提议将菌株MLFT归为一个新物种——嗜热食腐脱硫肠状菌的成员。
