Johansson J, Szyperski T, Wüthrich K
Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule-Hönggerberg, Zürich, Switzerland.
FEBS Lett. 1995 Apr 10;362(3):261-5. doi: 10.1016/0014-5793(95)00216-v.
The surfactant-associated polypeptide C (SP-C) is a 35-residue lipopolypeptide which is essential for the function of surfactants used for therapy of infant respiratory distress. Modeling based on the recently determined nuclear magnetic resonance (NMR) structure of native SP-C in an organic solvent showed that SP-C could readily insert into fluid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers. The present paper describes further physical-chemical studies of intact SP-C and its N-terminal 17-residue polypeptide fragment, depalmitoyl-SP-C(1-17), in the presence of dodecylphosphocholine micelles. The results obtained provide a link between the NMR solution structure and the behaviour of SP-C in an ordered lipid environment, and thus present new insights for rational design of SP-C analogs for therapeutic purposes.
表面活性剂相关多肽C（SP-C）是一种由35个氨基酸残基组成的脂多肽，对于用于治疗婴儿呼吸窘迫的表面活性剂的功能至关重要。基于最近在有机溶剂中测定的天然SP-C的核磁共振（NMR）结构进行的建模表明，SP-C能够很容易地插入到液态1,2-二棕榈酰-sn-甘油-3-磷酸胆碱双层膜中。本文描述了在十二烷基磷酸胆碱胶束存在下，对完整的SP-C及其N端17个氨基酸残基的多肽片段脱棕榈酰-SP-C(1-17)进行的进一步物理化学研究。所获得的结果在NMR溶液结构与SP-C在有序脂质环境中的行为之间建立了联系，从而为合理设计用于治疗目的的SP-C类似物提供了新的见解。
