Lahanas P N, Miyamoto M M, Bjorndal K A, Bolten A B
Department of Zoology, University of Florida, Gainesville 32611.
Genetica. 1994;94(1):57-66. doi: 10.1007/BF01429220.
The molecular evolution and population genetics of migratory green turtles (Chelonia mydas) in the Greater Caribbean were examined with mitochondrial DNA (mtDNA) control region I sequences. A total of 488 base positions (bp) per individual were aligned for 44 individuals from four nesting populations in Florida, Costa Rica, Aves Island (Venezuela), and Surinam. Twelve sequence polymorphisms were detected, representing ten transitions, one transversion, and one 10-bp repeat. Sequence analyses of within- and between-population diversity revealed a deep divergence between western and eastern Caribbean nesting colonies and an inverse relationship between reproductive female population size and mtDNA diversity. In small populations, genetic admixture was important to maintaining high diversity, whereas larger populations appear to have experienced historical bottlenecks or resulted from founder effects. Mitochondrial DNA sequences of the control region offer an order of magnitude greater resolution than restriction site data for addressing questions about mtDNA variation, both within and between populations of green turtles.
利用线粒体DNA（mtDNA）控制区I序列，对大加勒比地区洄游绿海龟（蠵龟）的分子进化和群体遗传学进行了研究。对来自佛罗里达州、哥斯达黎加、阿韦斯岛（委内瑞拉）和苏里南四个筑巢群体的44只个体，每个个体共488个碱基位置（bp）进行了比对。检测到12个序列多态性，包括10个转换、1个颠换和1个10-bp重复。群体内和群体间多样性的序列分析表明，西加勒比和东加勒比筑巢群体之间存在深度分化，且繁殖雌龟群体大小与mtDNA多样性呈负相关。在小群体中，基因混合对于维持高多样性很重要，而较大的群体似乎经历过历史瓶颈或由奠基者效应导致。控制区的线粒体DNA序列在解决绿海龟群体内和群体间mtDNA变异问题方面，比限制性位点数据的分辨率高出一个数量级。
