Kotyk A
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague.
Folia Microbiol (Praha). 1994;39(4):261-4. doi: 10.1007/BF02814309.
The transport rates of amino acids, ranging from L-Glu to L-Lys, uracil, adenine and sulfate and phosphate anions by Saccharomyces cerevisiae are greatly increased by preincubation with D-glucose in a nongrowth medium when a de novo synthesis of proteins takes place. In addition, some substrates, especially the inorganic anions, require the presence of glucose during their transport. This requirement has to do both with ongoing protein synthesis and degradation, as well as with providing energy and/or activating the plasma membrane H(+)-ATPase which supplies the protons to the H+ symports studied here.
当发生蛋白质从头合成时，酿酒酵母对从L-谷氨酸到L-赖氨酸、尿嘧啶、腺嘌呤以及硫酸根和磷酸根阴离子的转运速率，在非生长培养基中通过与D-葡萄糖预孵育而大幅增加。此外，一些底物，特别是无机阴离子，在其转运过程中需要葡萄糖的存在。这种需求与正在进行的蛋白质合成和降解有关，也与提供能量和/或激活质膜H(+)-ATP酶有关，该酶为本文研究的H+同向转运体提供质子。
