从尿路感染中分离出的铜绿假单胞菌菌株的特征。
Characteristics of Pseudomonas aeruginosa strains isolated from urinary tract infections.
作者信息
Puzová H, Siegfried L, Kmetová M, Durovicová J, Kerestesová A
机构信息
Institute of Medical Microbiology, Faculty of Medicine, Safárik University, Kosice, Slovakia.
出版信息
Folia Microbiol (Praha). 1994;39(4):337-41. doi: 10.1007/BF02814324.
Thirty-three uropathogenic strains of Pseudomonas aeruginosa were investigated for hemolytic activity in both bacterial broth culture filtrates and isolate lyzates, resistance to bactericidal activity of fresh human serum, resistance to six antibiotics and plasmid DNA profile. Twenty-four of the 33 (73%) bacterial filtrates showed lysis of rabbit erythrocytes, as did the three after guinea-pig erythrocyte treatment. Twelve of 33 isolate lysates showed in parallel lysis of both types of erythrocytes used. Serum resistance was found in 17 (52%) isolates, intermediate resistance in 15 (45 %) isolates and only one isolate showed serum sensitivity. Resistance to antibiotics was detected as follows (in %): tetracycline 94, kanamycin 79, chloramphenicol 76, septrin 73, ampicillin 64, streptomycin 45, gentamicin 18. None of the isolates investigated showed resistance to colistine. With the exception of one isolate, plasmid DNA was detected in all P. aeruginosa strains.
对33株尿路致病性铜绿假单胞菌进行了研究，检测其在细菌肉汤培养滤液和分离株裂解物中的溶血活性、对新鲜人血清杀菌活性的抗性、对六种抗生素的抗性以及质粒DNA图谱。33份细菌滤液中有24份（73%）显示能使兔红细胞裂解，用豚鼠红细胞处理后的3份滤液也有此现象。33份分离株裂解物中有12份对所用的两种红细胞均有平行裂解作用。17份（52%）分离株表现出抗血清性，15份（45%）分离株表现出中度抗性，只有1份分离株表现出对血清敏感。检测到的抗生素抗性如下（百分比）：四环素94、卡那霉素79、氯霉素76、复方新诺明73、氨苄西林64、链霉素45、庆大霉素18。所有被研究的分离株均未表现出对多粘菌素的抗性。除1份分离株外，在所有铜绿假单胞菌菌株中均检测到质粒DNA。
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