从大鼠多形核中性粒细胞（PMN）中清除摄入的32P标记大肠杆菌。一种方法的评估。
Elimination of ingested 32P-labelled E. coli from rat polymorphonuclear neutrophils (PMN). Evaluation of a method.
作者信息
Midtvedt T, Baardsen A
出版信息
Acta Pathol Microbiol Scand C. 1976 Apr;84(2):100-4. doi: 10.1111/j.1699-0463.1976.tb00005.x.
Abstract
A method to be used for in vitro assay of the elimination of ingested 32P-labelled E. coli from rat PMN monolayers has been evaluated. The rate of expulsion of bacterial label from phagocytes into the extracellular medium was found to range between 40 and 50% of the total uptake 180 min after termination of ingestion. Serum enhanced the cellular uptake of bacteria, but did not affect the rate of elimination. Disintegration of the phagocytes was not found to be a problem.
摘要
一种用于体外测定大鼠中性粒细胞单层中摄入的32P标记大肠杆菌清除情况的方法已得到评估。发现细菌标记从吞噬细胞排出到细胞外介质中的速率在摄入终止180分钟后占总摄取量的40%至50%之间。血清增强了细胞对细菌的摄取，但不影响清除速率。未发现吞噬细胞解体是一个问题。
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