Falk M C, Ng G, Zhang G Y, Fanning G C, Roy L P, Bannister K M, Thomas A C, Clarkson A R, Woodroffe A J, Knight J F
Renal Research Laboratories, Children's Hospital, Camperdown, New South Wales, Australia.
Kidney Int. 1995 Jan;47(1):177-85. doi: 10.1038/ki.1995.21.
We have studied renal biopsies from three groups of patients to determine if alpha beta T cells or gamma delta T cells are present, and whether their presence is correlated with disease progression in IgA nephropathy (IgAN). Group one comprised thin basement membrane disease biopsies (non-immunological control, N = 7); group two were patients with IgAN and stable renal function one year following biopsy (stable, N = 7); and group three were IgAN patients with rapidly declining renal function after one year (progressive, N = 7). Immunohistochemical staining using monoclonal antibodies (CD3, TcR beta, TcR delta) and molecular studies utilizing polymerase chain reaction amplification of cDNA transcribed from biopsy RNA, with primers specific for either the alpha beta TcR or gamma delta TcR, were undertaken. On immunohistochemistry a significant increase in CD3 + cells in progressive biopsies was seen (vs. control P = 0.002, vs. stable P = 0.002). The progressive biopsies infiltrate consisted of both alpha beta TcR (vs. control P = 0.001, vs. stable P = 0.003) and gamma delta TcR cells (vs. control P = 0.01). The RNA study demonstrated an increase in TcR C alpha transcription in the progressive (vs. control P = 0.003) biopsies. Increased TcR C delta transcription was seen in the progressive group (vs. control P = 0.01, vs. stable P = 0.02). We confirm that the presence of lymphocytes in IgAN biopsies predicts progressive disease. While alpha beta T cells are found in both stable and progressive disease, the presence of gamma delta T cells is only associated with progressive IgAN.
我们研究了三组患者的肾活检样本，以确定是否存在αβT细胞或γδT细胞，以及它们的存在是否与IgA肾病（IgAN）的疾病进展相关。第一组包括薄基底膜肾病活检样本（非免疫对照，N = 7）；第二组是活检后一年肾功能稳定的IgAN患者（稳定组，N = 7）；第三组是活检后一年肾功能迅速下降的IgAN患者（进展组，N = 7）。采用单克隆抗体（CD3、TcRβ、TcRδ）进行免疫组织化学染色，并利用聚合酶链反应扩增活检RNA转录的cDNA进行分子研究，所用引物分别针对αβTcR或γδTcR。免疫组织化学显示，进展性活检样本中CD3 +细胞显著增加（与对照组相比P = 0.002，与稳定组相比P = 0.002）。进展性活检样本中的浸润细胞包括αβTcR细胞（与对照组相比P = 0.001，与稳定组相比P = 0.003）和γδTcR细胞（与对照组相比P = 0.01）。RNA研究表明，进展性活检样本中TcR Cα转录增加（与对照组相比P = 0.003）。进展组中可见TcR Cδ转录增加（与对照组相比P = 0.01，与稳定组相比P = 0.02）。我们证实，IgAN活检样本中淋巴细胞的存在预示着疾病进展。虽然在稳定期和进展期疾病中均发现了αβT细胞，但γδT细胞的存在仅与进展性IgAN相关。
