Abu-Elheiga L, Jayakumar A, Baldini A, Chirala S S, Wakil S J
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030, USA.
Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):4011-5. doi: 10.1073/pnas.92.9.4011.
We have cloned and sequenced the cDNA coding for human HepG2 acetyl-CoA carboxylase (ACC; EC 6.4.1.2). The sequence has an open reading frame of 7038 bp that encode 2346 amino acids (M(r), 264,737). The C-terminal 2.6-kb sequence is very different from that recently reported for human ACC (Ha, J., Daniel, S., Kong, I.-S., Park, C.-K., Tae, H.-J. & Kim, K.-H. [1994] Eur. J. Biochem. 219, 297-306). Northern blot analysis revealed that the ACC mRNA is approximately 10 kb in size and that its level varies among the tissues tested. Evidence is presented to show that the human ACC gene is 200-480 kbp in size and maps to chromosome 17q12. We also provide evidence for the presence of another ACC-like gene with similarly sized mRNA but tissue-specific expression different from that of the ACC gene reported herein. That this second ACC-like gene encodes the 280-kDa carboxylase is not ruled out.
我们已经克隆并测序了编码人HepG2乙酰辅酶A羧化酶（ACC；EC 6.4.1.2）的cDNA。该序列有一个7038 bp的开放阅读框，编码2346个氨基酸（分子量为264,737）。其C末端2.6 kb的序列与最近报道的人ACC序列（Ha, J., Daniel, S., Kong, I.-S., Park, C.-K., Tae, H.-J. & Kim, K.-H. [1994] Eur. J. Biochem. 219, 297 - 306）有很大不同。Northern印迹分析显示，ACC mRNA大小约为10 kb，其水平在测试的组织中有所不同。有证据表明，人ACC基因大小为200 - 480 kbp，定位于染色体17q12。我们还提供了证据，证明存在另一个ACC样基因，其mRNA大小相似，但组织特异性表达与本文报道的ACC基因不同。不排除这个第二个ACC样基因编码280 kDa羧化酶。
