Widmer J, Fassihi K S, Schlichter S C, Wheeler K S, Crute B E, King N, Nutile-McMenemy N, Noll W W, Daniel S, Ha J, Kim K H, Witters L A
Department of Medicine, Dartmouth Medical School, Hanover NH 03755, USA.
Biochem J. 1996 Jun 15;316 ( Pt 3)(Pt 3):915-22. doi: 10.1042/bj3160915.
Acetyl-CoA carboxylase (ACC), an important enzyme in fatty acid biosynthesis and a regulator of fatty acid oxidation, is present in at least two isoenzymic forms in rat and human tissues. Previous work has established the existence of a 265,000 Da enzyme in both the rat and human (RACC265; HACC265) and a higher-molecular-mass species (275,000-280,000 Da) in the same species (RACC280; HACC275). An HACC265 gene has previously been localized to chromosome 17. In the present study, we report cloning of a partial-length human cDNA sequence which appears to correspond to HACC275 and its rat homologue, RACC280, as judged by mRNA tissue distribution and cell-specific regulation of mRNA/protein expression. The gene encoding this isoenzymic form of ACC has been localized to the long arm of human chromosome 12. Thus, ACC is represented in a multigene family in both rodents and humans. The newly discovered human gene and its rat homologue appear to be under different regulatory control to the HACC265 gene, as judged by tissue-specific expression in vivo and by independent modulation in cultured cells in vitro.
乙酰辅酶A羧化酶（ACC）是脂肪酸生物合成中的一种重要酶，也是脂肪酸氧化的调节因子，在大鼠和人类组织中至少以两种同工酶形式存在。先前的研究已证实大鼠和人类体内均存在一种265,000道尔顿的酶（RACC265；HACC265），且同一物种中还存在一种分子量更高的酶（275,000 - 280,000道尔顿）（RACC280；HACC275）。先前已将HACC265基因定位到17号染色体。在本研究中，我们报告了一个部分长度的人类cDNA序列的克隆，根据mRNA组织分布以及mRNA/蛋白质表达的细胞特异性调控判断，该序列似乎对应于HACC275及其大鼠同源物RACC280。编码这种ACC同工酶形式的基因已定位到人类12号染色体长臂。因此，ACC在啮齿动物和人类中均由一个多基因家族所代表。根据体内组织特异性表达以及体外培养细胞中的独立调控判断，新发现的人类基因及其大鼠同源物似乎受到与HACC265基因不同的调控。
