Bekana M, Jonsson P, Ekman T, Kindahl H
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
Zentralbl Veterinarmed A. 1994 Nov;41(9):663-70. doi: 10.1111/j.1439-0442.1994.tb00134.x.
Eleven Swedish postpartum cows with retained fetal membranes (RFM) were studied to determine the intrauterine bacterial flora. Bacteriological examination was performed from twice weekly uterine biopsies. A total of 161 biopsies were collected during the first 8 weeks postpartum of which 82 (50.9%) were found with bacterial growth. Seventy-one of the 82 bacteria-positive biopsies (86.6%) showed mixed infections whereas the remaining 11 (13.4%) were pure cultures. Generally, a total of 322 isolates belonging to 12 different genera of bacteria, 6 facultative and 6 obligate anaerobic pathogens were identified. Mixed infections were most frequent for Actinomyces pyogenes together with obligate anaerobic bacteria, especially Bacteroides levii/spp. and Fusobacterium necrophorum. All of the studied cows had an infection that involved the first two genera of bacteria, whereas F. necrophorum was found in 8 of the 11 animals. The present work suggests that a possible pathogenic synergism between A. pyogenes and the two main Gram-negative anaerobes might have caused early endometritis and/or persistent infection.
对11头患有胎膜残留（RFM）的瑞典产后奶牛进行了研究，以确定子宫内的细菌菌群。每周两次从子宫活检组织进行细菌学检查。在产后的前8周共收集了161份活检组织，其中82份（50.9%）发现有细菌生长。82份细菌阳性活检组织中的71份（86.6%）显示为混合感染，其余11份（13.4%）为纯培养物。一般来说，共鉴定出322株属于12个不同细菌属的菌株，其中6种为兼性厌氧菌，6种为专性厌氧菌病原体。化脓放线菌与专性厌氧菌，尤其是轻发酵拟杆菌/某些种和坏死梭杆菌的混合感染最为常见。所有研究的奶牛都感染了前两种细菌，而11头动物中有8头发现有坏死梭杆菌。目前的研究表明，化脓放线菌与两种主要的革兰氏阴性厌氧菌之间可能存在致病协同作用，这可能导致了早期子宫内膜炎和/或持续性感染。
