Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie de Monastir, Monastir 5000, Tunisie.
BMC Complement Altern Med. 2012 Sep 13;12:153. doi: 10.1186/1472-6882-12-153.
Plants play a significant role in maintaining human health and improving the quality of human life. They serve humans well as valuable components of food, as well as in cosmetics, dyes, and medicines. In fact, many plant extracts prepared from plants have been shown to exert biological activity in vitro and in vivo. The present study explored antioxidant and antigenotoxic effects of Daphne gnidium leaf extracts.
The genotoxic potential of petroleum ether, chloroform, ethyl acetate, methanol and total oligomer flavonoid (TOF) enriched extracts from leaves of Daphne gnidium, was assessed using Escherichia coli PQ37. Likewise, the antigenotoxicity of the same extracts was tested using the "SOS chromotest test". Antioxidant activities were studied using non enzymatic and enzymatic method: NBT/Riboflavine and xantine oxidase.
None of the different extracts produced a genotoxic effect, except TOF extract at the lowest tested dose. Our results showed that D. gnidium leaf extracts possess an antigenotoxic effect against the nitrofurantoin a mutagen of reference. Ethyl acetate and TOF extracts were the most effective in inhibiting xanthine oxidase activity. While, methanol extract was the most potent superoxide scavenger when tested with the NBT/Riboflavine assay.
The present study has demonstrated that D. gnidium leaf extract possess antioxidant and antigenotoxic effects. These activities could be ascribed to compounds like polyphenols and flavonoid. Further studies are required to isolate the active molecules.
植物在维持人类健康和提高人类生活质量方面发挥着重要作用。它们作为食物的有价值成分，以及在化妆品、染料和药物中，都为人类提供了很好的服务。事实上，许多从植物中提取的植物提取物已被证明在体外和体内具有生物活性。本研究探讨了瑞香狼毒叶提取物的抗氧化和抗原毒性作用。
使用大肠杆菌 PQ37 评估石油醚、氯仿、乙酸乙酯、甲醇和总低聚黄酮（TOF）富集提取物对 Daphne gnidium 叶的遗传毒性潜力。同样，使用“SOS 显色试验”测试了相同提取物的抗原毒性。使用非酶和酶方法研究了抗氧化活性：NBT/核黄素和黄嘌呤氧化酶。
除了 TOF 提取物在最低测试剂量外，不同提取物均未产生遗传毒性作用。我们的结果表明，D. gnidium 叶提取物对硝基呋喃妥因这种参考诱变剂具有抗原毒性作用。乙酸乙酯和 TOF 提取物对黄嘌呤氧化酶活性的抑制作用最强。而甲醇提取物在 NBT/核黄素测定中是最强的超氧化物清除剂。
本研究表明 D. gnidium 叶提取物具有抗氧化和抗原毒性作用。这些活性可能归因于多酚和类黄酮等化合物。需要进一步研究以分离活性分子。
