Batliwala H, Somasundaram T, Uzgiris E E, Makowski L
Department of Physics, Boston University, MA 02215, USA.
Biochem J. 1995 Apr 15;307 ( Pt 2)(Pt 2):433-8. doi: 10.1042/bj3070433.
Human erythrocytes were exposed to high concentrations of methane and nitrogen through the application of elevated partial pressures of these gas molecules. Cell leakage (haemolysis) was measured for cells exposed to these gases under a wide range of experimental conditions. Application of methane produces haemolysis at pressures far below the hydrostatic pressures known to disrupt membrane or protein structure. The effects of changes in buffer, temperature, diffusion rate and detergents were studied. Methane acts co-operatively with detergents to produce haemolysis at much lower detergent concentration than is required in the absence of methane or in the presence of nitrogen. At sufficiently high concentrations of methane, all cells are haemolysed. Increased temperature enhances the effect. Methane produces 50% haemolysis at a concentration of about 0.33 M compared with about 7.5 M methanol required for the same degree of haemolysis.
通过施加这些气体分子的较高分压，将人类红细胞暴露于高浓度的甲烷和氮气中。在广泛的实验条件下，测量暴露于这些气体的细胞的渗漏情况（溶血）。在远低于已知会破坏膜或蛋白质结构的静水压力下，施加甲烷会产生溶血现象。研究了缓冲液、温度、扩散速率和去污剂变化的影响。甲烷与去污剂协同作用，在比不存在甲烷或存在氮气时所需浓度低得多的去污剂浓度下产生溶血。在足够高的甲烷浓度下，所有细胞都会溶血。温度升高会增强这种效应。与产生相同程度溶血所需的约7.5 M甲醇相比，甲烷在约0.33 M的浓度下产生50%的溶血。
