Hentunen T A, Lakkakorpi P T, Tuukkanen J, Lehenkari P P, Sampath T K, Väänänen H K
Department of Anatomy, University of Oulu, Finland.
Biochem Biophys Res Commun. 1995 Apr 17;209(2):433-43. doi: 10.1006/bbrc.1995.1521.
Recombinant human OP-1 stimulated the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells (MNCs) significantly and in a dose-dependent manner in rat bone marrow cell culture. Newly formed MNCs either induced by hOP-1 alone or with 1,25(OH)2D3 were also positive for vitronectin receptor and carbonic anhydrase II. Moreover, OP-1 markedly increased the capacity of 1,25(OH)2D3 to induce osteoclast-like cell formation and bone resorption in bone marrow cultures. 25 pg/ml of calcitonin significantly inhibited both OP-1- and vitamin D3-induced TRAP-positive MNC-formation in marrow cultures, indicating that in both cases the MNC formation was calcitonin sensitive. OP-1 at 5-100 ng/ml did not have any significant effect on bone resorption as studied by pit formation assay. These studies that OP-1 in concert with 1,25(OH)2D3 could have an important role in bone remodeling by exhibiting its effects not only on osteoblast growth and differentiation but also on the recruitment of osteoclasts.
重组人骨形态发生蛋白-7（OP-1）在大鼠骨髓细胞培养中能显著且呈剂量依赖性地刺激抗酒石酸酸性磷酸酶（TRAP）阳性多核细胞（MNCs）的形成。单独由hOP-1或与1,25(OH)₂D₃共同诱导形成的新MNCs对玻连蛋白受体和碳酸酐酶II也呈阳性。此外，OP-1显著增强了1,25(OH)₂D₃在骨髓培养中诱导破骨细胞样细胞形成和骨吸收的能力。25 pg/ml的降钙素显著抑制了骨髓培养中OP-1和维生素D₃诱导的TRAP阳性MNCs的形成，表明在这两种情况下MNCs的形成对降钙素敏感。通过陷窝形成试验研究发现，5 - 100 ng/ml的OP-1对骨吸收没有任何显著影响。这些研究表明，OP-1与1,25(OH)₂D₃协同作用，不仅对成骨细胞的生长和分化有影响，而且对破骨细胞的募集也有作用，可能在骨重塑中发挥重要作用。
