Chuang L F, Chuang T K, Killam K F, Chuang A J, Kung H F, Yu L, Chuang R Y
Department of Medical Pharmacology and Toxicology, University of California, Davis 95616.
Biochem Biophys Res Commun. 1994 Aug 15;202(3):1291-9. doi: 10.1006/bbrc.1994.2071.
Previous studies have shown that morphine stimulates simian immunodeficiency virus (SIV) replication in SIV-infected human CEM x174 cells as well as in monkey lymphocytes through a mechanism of delaying the lysis of infected cells (Biochem. Biophys. Res. Commun. 195:1165-1173, 1993). The present study describes the identification of brain-like opioid receptor sequences in RNA transcripts of both CEM x174 cells and monkey lymphocytes. Study of the gene sequence of a lymphocyte opioid receptor encompassing the third transmembrane domain and the third cytoplasmic loop indicates a 96% homology in amino acid composition to the delta opioid receptor in brain cells. Expression of such an opioid receptor sequence in lymphocytic cells is constitutive, since it could be detected in both saline-treated and morphine-treated monkeys as well as in morphine-treated monkeys after detoxification.
先前的研究表明，吗啡通过延迟感染细胞裂解的机制，刺激猿猴免疫缺陷病毒（SIV）在感染SIV的人类CEM x174细胞以及猴淋巴细胞中的复制（《生物化学与生物物理研究通讯》195:1165 - 1173，1993年）。本研究描述了在CEM x174细胞和猴淋巴细胞的RNA转录本中鉴定出脑样阿片受体序列。对包含第三个跨膜结构域和第三个细胞质环的淋巴细胞阿片受体基因序列的研究表明，其氨基酸组成与脑细胞中的δ阿片受体有96%的同源性。这种阿片受体序列在淋巴细胞中的表达是组成性的，因为在生理盐水处理的猴子、吗啡处理的猴子以及戒毒后的吗啡处理猴子中均能检测到。
