Clark J M, Glagov S
Br J Exp Pathol. 1976 Feb;57(1):129-35.
Perfusion fixation at physiological pressures, careful tissue handling, adequate drying and reduced beam exposure time eliminated many of the intimal surface projections, ridges and bridges which have been taken for normal structures on scanning electron microscopy. In the normal distended vessel, ovoid endothelial nuclei bulged into the lumen with their major axes aligned in the direction of flow: adjacent cell margins overlapped consistently in the direction of flow, with each cell overlapping the edge of its downstream neighbour. Regular longitudinal furrows associated with undulations of the internal elastin lamina were entirely eliminated from elastic arteries when distending pressures exceeded diastolic levels during fixation.
在生理压力下进行灌注固定、小心处理组织、充分干燥并缩短电子束照射时间，消除了许多在扫描电子显微镜下被视为正常结构的内膜表面突起、嵴和桥。在正常扩张的血管中，椭圆形的内皮细胞核向管腔内突出，其长轴与血流方向一致：相邻细胞边缘在血流方向上持续重叠，每个细胞与其下游相邻细胞的边缘重叠。当在固定过程中扩张压力超过舒张压水平时，弹性动脉中与内弹性膜波动相关的规则纵向沟完全消失。
