Ongrádi J, Szilágyi F J, Laird M H, Ceccherini-Nelli L, Pistello M, Bendinelli M
Glasgow-i Egyetem, Virológiai Intézet, Nagy-Britannia.
Orv Hetil. 1993 Apr 11;134(15):803-9.
The hallmark of AIDS is the gradual loss of CD4+ T-lymphocytes, in spite of their infection in low ratio. The pathomechanism is hardly known, therefore, the production of HIV-1 and certain aspects of cell death were studied. Infectivity was decreased by the acidification of culture media. C8166 cells transformed by HTLV-I and exhibiting features of both immature T lymphocytes and myeloid cells, produced transient protoplasmic surface extrusions, similar to the hairy cell leukemia. These can have a role in the direct cell-to-cell spread of HIV-1. Polarization of nuclei and cell organelles as well as sites of virus budding during syncytium formation resembled the directed lymphokine secretion. Both cell membrane and intravacuolar buddings were characteristic. Abnormal virus particles also were seen. Certain morphological signs resembled apoptosis. Fibroblast cultures in the presence of HIV-1 infected lymphoid cells were arrested in growth and underwent cell death without syncytium formation. The results draw attention to the faster development of AIDS in the case of HIV-1 infection of precursor immune cells. Double infection by HTLV and HIV-1 can result in atypical leukemias.
艾滋病的标志是CD4+ T淋巴细胞逐渐减少，尽管其感染率较低。其发病机制尚不清楚，因此，对HIV-1的产生及细胞死亡的某些方面进行了研究。培养基酸化会降低感染性。由HTLV-I转化的C8166细胞兼具未成熟T淋巴细胞和髓细胞的特征，会产生短暂的原生质表面突起，类似于毛细胞白血病。这些突起可能在HIV-1的直接细胞间传播中起作用。在形成合胞体期间，细胞核和细胞器的极化以及病毒出芽位点类似于定向淋巴因子分泌。细胞膜和液泡内出芽均具有特征性。还可见到异常病毒颗粒。某些形态学迹象类似于细胞凋亡。在存在感染HIV-1的淋巴细胞的情况下，成纤维细胞培养物生长停滞并在未形成合胞体的情况下发生细胞死亡。结果表明，在前体免疫细胞感染HIV-1的情况下，艾滋病的发展更快。HTLV和HIV-1双重感染可导致非典型白血病。
