Dekker A, Wensvoort G, Terpstra C
Department of Virology, Institute for Animal Science and Health, Lelystad, Netherlands.
Vet Microbiol. 1995 Dec;47(3-4):317-29. doi: 10.1016/0378-1135(95)00116-6.
Antigenic differences between pestivirus isolates of ruminant and porcine origin were characterized by neutralization assays. First, six different clusters of pestiviruses were identified by clustering cross-neutralization results of 13 pestivirus strains tested against 23 sera. Cluster I consisted of four strains of bovine viral diarrhoea virus (BVDV) of bovine origin and two BVDV isolates of porcine origin. Cluster II consisted of one sheep isolate and two porcine BDV isolates. Cluster III consisted of one classical swine fever virus strain and cluster IV, V, and VI each consisted of one strain isolated from a giraffe, a deer, and a pig. After the clusters were identified, one-way neutralization tests were used to test a total of 45 pestivirus isolates. Although the same six groups were found, results of some individual strains differed from previous cross-neutralization results and the results obtained by typing with monoclonal antibodies. The discrepancy between one way neutralization tests and cross-neutralisation tests is demonstrated clearly by recalculation of the cross-neutralization results without the classical swine fever sera. We conclude that neutralization tests are only suitable to characterize antigenic differences when virus strains are tested in a cross-neutralization test.
通过中和试验对反刍动物源和猪源瘟病毒分离株之间的抗原差异进行了表征。首先，通过对13种瘟病毒株针对23份血清的交叉中和结果进行聚类分析，鉴定出了六种不同的瘟病毒簇。簇I由四株牛源牛病毒性腹泻病毒（BVDV）和两株猪源BVDV分离株组成。簇II由一株绵羊分离株和两株猪源BVDV分离株组成。簇III由一株经典猪瘟病毒株组成，簇IV、V和VI各由一株分别从长颈鹿、鹿和猪分离得到的毒株组成。在鉴定出这些簇之后，使用单向中和试验对总共45种瘟病毒分离株进行了检测。虽然发现了相同的六组，但一些个别毒株的结果与先前的交叉中和结果以及用单克隆抗体分型获得的结果不同。在不使用经典猪瘟血清重新计算交叉中和结果时，清楚地证明了单向中和试验与交叉中和试验之间的差异。我们得出结论，只有在交叉中和试验中检测病毒株时，中和试验才适合表征抗原差异。
