Pemberton J M, Slate J, Bancroft D R, Barrett J A
Department of Genetics, University of Cambridge, UK.
Mol Ecol. 1995 Apr;4(2):249-52. doi: 10.1111/j.1365-294x.1995.tb00214.x.
While genotyping wild red deer (Cervus elaphus) at microsatellite loci for paternity assignment, we found three loci (MAF65, BOVIRBP and CelJP23) with segregating nonamplifying alleles. Nonamplifying alleles were detected through mismatches between known mother-offspring pairs and by significant deviations from Hardy-Weinberg equilibria. In a wide range of molecular ecology application, and especially in parentage assignment, the possible existence of undetectable alleles must be taken into account; this may be particularly important for microsatellite data.
在对野生马鹿（ Cervus elaphus）进行微卫星基因座基因分型以确定父系关系时，我们发现了三个基因座（MAF65、BOVIRBP和CelJP23）存在分离的非扩增等位基因。通过已知母子对之间的不匹配以及与哈迪-温伯格平衡的显著偏差检测到了非扩增等位基因。在广泛的分子生态学应用中，尤其是在亲子关系鉴定中，必须考虑到可能存在无法检测到的等位基因；这对于微卫星数据可能尤为重要。
