Slate J, Coltman D W, Goodman S J, MacLean I, Pemberton J M, Williams J L
University of Edinburgh, UK.
Anim Genet. 1998 Aug;29(4):307-15. doi: 10.1046/j.1365-2052.1998.00347.x.
We tested 174 bovine microsatellite primer pairs for use in a primitive breed of sheep and two species of deer. Of 173 markers, 127 (73.4%) gave a product in Soay sheep (Ovis aries) of which 54 (42.5%) were polymorphic. One hundred and twenty-nine of 174 (74.1%) markers gave a product in red deer (Cervus elaphus) of which 72 (55.8%) were polymorphic. In sika deer (Cervus nippon) 126 of 171 (73.7%) microsatellite primers gave a product with 47 (37.3%) polymorphic. The proportion of bovine microsatellite loci conserved across artiodactyl species was significantly greater in this study than previously reported. Reasons for this high degree of microsatellite conservation are discussed. We suggest that a high resolution comparative map of the artiodactyls can be constructed using microsatellites.
我们对174对牛微卫星引物进行了测试，以用于一个原始品种的绵羊和两种鹿。在173个标记中，127个（73.4%）在索艾羊（绵羊）中扩增出产物，其中54个（42.5%）具有多态性。174个标记中的129个（74.1%）在马鹿中扩增出产物，其中72个（55.8%）具有多态性。在梅花鹿中，171个微卫星引物中的126个（73.7%）扩增出产物，其中47个（37.3%）具有多态性。本研究中跨偶蹄目物种保守的牛微卫星基因座比例显著高于先前报道。讨论了这种高度微卫星保守的原因。我们建议可以使用微卫星构建偶蹄目的高分辨率比较图谱。
