Dhont M, de Gezelle H, Vandekerckhove D
Clin Endocrinol (Oxf). 1976 Mar;5(2):175-80. doi: 10.1111/j.1365-2265.1976.tb02829.x.
The effect of clomiphene (100 mg daily for 10 days) and ethinyl oestradiol (100 mug daily for 10 days) on the gonadotrophin response to synthetic LHRH has been investigated in two groups of five normal males. A third group of five men served as control group. LHRH, 25 mug, was injected intravenously on days 0, 4, 7 and 10 and the response of serum LH and FSH was monitored by radioimmunoassay. In contrast to the wide inter-individual variation of the response pattern, the intra-individual variation of the response to LHRH in the control group was small. Clomiphene induced a significant elevation of the baseline levels of LH and FSH after a few days of treatment; the pituitary responsiveness to LHRH, however, was significantly reduced. Oestrogen treatment resulted in a uniform suppression of both basal gonadotrophin levels and pituitary responsiveness. The decreased gonadotrophin response to LHRH during clomiphene treatment is thought to be caused by a relative and temporary pituitary depletion of the releasable gonadotrophin content. Although the suppression of LH and FSH response during oestrogen treatment may point to a direct inhibitory effect of oestrogen on pituitary gonadotrophin release, an indirect hypothalamic pathway, through suppression of endogenous LHRH, cannot be ruled out.
已在两组各五名正常男性中研究了克罗米芬（每日100毫克，共10天）和炔雌醇（每日100微克，共10天）对促性腺激素对合成促黄体生成素释放激素（LHRH）反应的影响。第三组五名男性作为对照组。在第0、4、7和10天静脉注射25微克LHRH，并通过放射免疫测定法监测血清促黄体生成素（LH）和促卵泡生成素（FSH）的反应。与反应模式的个体间广泛差异相反，对照组中对LHRH反应的个体内差异较小。克罗米芬在治疗几天后可使LH和FSH的基线水平显著升高；然而，垂体对LHRH的反应性显著降低。雌激素治疗导致基础促性腺激素水平和垂体反应性均受到一致抑制。克罗米芬治疗期间促性腺激素对LHRH反应的降低被认为是由于可释放促性腺激素含量的相对和暂时垂体耗竭所致。尽管雌激素治疗期间LH和FSH反应的抑制可能表明雌激素对垂体促性腺激素释放有直接抑制作用，但通过抑制内源性LHRH的间接下丘脑途径也不能排除。
