Salman N H, Fuhrman B P, Steinhorn D M, Papo M C, Hernan L J, Leach C L, Fischer J E
School of Medicine and Biomedical Sciences, State University of New York at Buffalo, USA.
Crit Care Med. 1995 May;23(5):919-24. doi: 10.1097/00003246-199505000-00021.
To determine whether oxygenation and lung mechanics are preserved during perfluorocarbon associated gas exchange of 24 hrs duration and after evaporation of perfluorocarbon.
Prospective, experimental animal trials.
Animal laboratory in a university setting.
Ten normal, neonatal piglets weighing 2.5 to 4.5 kg.
Ten piglets were anesthetized with fentanyl (25 micrograms/kg/hr), paralyzed with metocurine iodide (0.3 mg/kg) and placed on volume regulated continuous positive pressure breathing instituted at an FIO2 setting of 1.0, tidal volume of 15 mL/kg, respiratory rate of 25 breaths/min and positive end-expiratory pressure of 4 cm H2O. Perfluorocarbon associated gas exchange was initiated by intratracheal instillation of perflouorooctylbromide (30 mL/kg) followed by gas ventilation at the same settings. Evaporative losses were replaced by intratracheal instillation of 2.5 mL/kg/hr of perfluorocarbon. In one group of five piglets, evaporative losses were replaced for 24 hrs until the end of the study. In the other group of five piglets, replacement of perfluorocarbon was discontinued after 2 hrs, although gas ventilation was continued for 24 hrs. Blood gases and lung mechanics were measured in both groups. Histologic evaluation of lungs from both groups of animals was performed.
Airway pressures and blood gases were stable throughout the 24-hr study period in both groups. Airway pressures in the evaporative group increased as evaporation of perfluorocarbon neared completion. There was no hemodynamic deterioration during the 24-hr study period. Histology showed good preservation of lung architecture in both groups.
Perfluorocarbon associated gas exchange was safe and effective in normal piglets for a period of 24 hrs. Evaporation of perfluorocarbon and resumption of continuous positive pressure breathing was well tolerated.
确定在持续24小时的全氟化碳相关气体交换过程中以及全氟化碳蒸发后，氧合作用和肺力学是否得以维持。
前瞻性实验动物试验。
大学环境中的动物实验室。
10只体重2.5至4.5千克的正常新生仔猪。
10只仔猪用芬太尼（25微克/千克/小时）麻醉，用碘化甲筒箭毒（0.3毫克/千克）使其麻痹，并置于容量控制的持续正压通气下，设置吸入氧浓度为1.0，潮气量为15毫升/千克，呼吸频率为25次/分钟，呼气末正压为4厘米水柱。通过气管内注入全氟辛基溴（30毫升/千克）启动全氟化碳相关气体交换，然后在相同设置下进行气体通气。通过气管内注入2.5毫升/千克/小时的全氟化碳来补充蒸发损失。在一组5只仔猪中，补充蒸发损失持续24小时直至研究结束。在另一组5只仔猪中，2小时后停止补充全氟化碳，尽管气体通气持续24小时。两组均测量血气和肺力学。对两组动物的肺进行组织学评估。
两组在整个24小时研究期间气道压力和血气均稳定。随着全氟化碳蒸发接近完成，蒸发组的气道压力升高。在24小时研究期间没有血流动力学恶化。组织学显示两组肺结构均保存良好。
全氟化碳相关气体交换在正常仔猪中24小时内是安全有效的。全氟化碳的蒸发和持续正压呼吸的恢复耐受性良好。
