Hughes D A, Fraser I P, Gordon S
Sir William Dunn School of Pathology, Oxford, UK.
Immunol Lett. 1994 Dec;43(1-2):7-14. doi: 10.1016/0165-2478(94)00143-x.
In the absence of divalent cations M phi lose their spread morphology but remain adherent to tissue culture treated plastic. We have exploited this property of M phi adhesion in vitro, to isolate a rat monoclonal 2F8, which totally inhibits the divalent cation-independent adhesion of M phi to tissue culture treated plastic and is partly responsible for the trypsin-resistant adhesion of M phi to the same substratum. Immunoprecipitation from macrophages and stably transfected Chinese Hamster Ovary Cells revealed that the antigen recognised by monoclonal antibody 2F8 is identical to the murine macrophage scavenger receptor. Macrophage scavenger receptors are trimeric integral membrane glycoproteins which have been implicated in various macrophage functions including uptake of oxidised lipoprotein and the formation of foam cells in atherosclerotic lesions. We have used monoclonal antibody 2F8 to explore the expression of murine macrophage scavenger receptor in lymphoid and non-lymphoid organs of the normal adult mouse and to examine the contribution of macrophage scavenger receptor to macrophage adhesion within tissues.
在缺乏二价阳离子的情况下，巨噬细胞（M phi）会失去其伸展形态，但仍能黏附于经组织培养处理的塑料表面。我们利用巨噬细胞在体外的这种黏附特性，分离出一种大鼠单克隆抗体2F8，它能完全抑制巨噬细胞与经组织培养处理的塑料表面的非二价阳离子依赖性黏附，并且部分介导巨噬细胞与同一基质的胰蛋白酶抗性黏附。对巨噬细胞和稳定转染的中国仓鼠卵巢细胞进行免疫沉淀分析表明，单克隆抗体2F8识别的抗原与小鼠巨噬细胞清道夫受体相同。巨噬细胞清道夫受体是三聚体整合膜糖蛋白，参与多种巨噬细胞功能，包括摄取氧化型脂蛋白以及在动脉粥样硬化病变中形成泡沫细胞。我们使用单克隆抗体2F8来研究正常成年小鼠淋巴和非淋巴器官中鼠巨噬细胞清道夫受体的表达，并探讨巨噬细胞清道夫受体对组织内巨噬细胞黏附的作用。
