人胎盘和蜕膜组织中白细胞介素-8信使核糖核酸及蛋白的检测与定位
Detection and localization of interleukin-8 mRNA and protein in human placenta and decidual tissues.
作者信息
Saito S, Kasahara T, Sakakura S, Umekage H, Harada N, Ichijo M
机构信息
Department of Obstetrics and Gynecology, Nara Medical University, Japan.
出版信息
J Reprod Immunol. 1994 Dec;27(3):161-72. doi: 10.1016/0165-0378(94)90001-9.
It has been reported that interleukin-8 (IL-8) is secreted from the placental and decidual tissues and that IL-8 levels in the amniotic fluids are significantly elevated by chorioamnionitis or labor pain. The present study was aimed at defining the localization of IL-8 mRNA as well as IL-8 protein at the feto-maternal interface using in situ hybridization and immunohistochemical staining. Both IL-8 mRNA and protein were localized in cytotrophoblast, syncytiotrophoblast and Hofbauer cells of the placenta, decidual stromal cells, decidual lymphocytes and endometrial gland cells. IL-8 secretion from glandular cells has not previously been reported. In addition, we confirmed IL-8 mRNA expression and secretion of IL-8 by an endometrial cancer cell line (Ishikawa) using the reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) methods, respectively.
据报道，白细胞介素-8（IL-8）由胎盘和蜕膜组织分泌，且羊水中的IL-8水平会因绒毛膜羊膜炎或产痛而显著升高。本研究旨在利用原位杂交和免疫组化染色确定IL-8 mRNA以及IL-8蛋白在母胎界面的定位。IL-8 mRNA和蛋白均定位于胎盘的细胞滋养层、合体滋养层和霍夫鲍尔细胞、蜕膜基质细胞、蜕膜淋巴细胞及子宫内膜腺细胞中。此前尚未有腺细胞分泌IL-8的报道。此外，我们分别使用逆转录聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）方法，证实了子宫内膜癌细胞系（石川细胞）中IL-8 mRNA的表达及IL-8的分泌。
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