在来源于生殖嵴的基质细胞上培养的鸡原始生殖细胞的增殖。

Proliferation of chick primordial germ cells cultured on stroma cells from the germinal ridge.

作者信息

Chang I K, Tajima A, Chikamune T, Ohno T

机构信息

RIKEN Cell Bank, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.

出版信息

Cell Biol Int. 1995 Feb;19(2):143-9. doi: 10.1006/cbir.1995.1055.

DOI:10.1006/cbir.1995.1055

PMID:7742779

Abstract

Fluorescent reagent-labelled PGCs isolated from the blood of 2-day-old chick embryos were cultured on stroma cells derived from 5-day-old germinal ridge in Medium 199 supplemented with 10% FBS, human IGF-1, bovine FGF-b, and murine LIF. In 7 experiments, the number of MCs increased by an average of 4.8 fold in 4 days. Intrinsic PGCs in the 5-day embryonic germinal ridge were observed loosely attached to the stroma cells, and they also increased 3.8 fold during culture for 4 days. These results indicate the possibility of applying this culture method to the production of transgenic chickens.

摘要

从2日龄鸡胚血液中分离出的荧光试剂标记的原始生殖细胞（PGCs），在添加了10%胎牛血清（FBS）、人胰岛素样生长因子-1（IGF-1）、牛碱性成纤维细胞生长因子（FGF-b）和小鼠白血病抑制因子（LIF）的199培养基中，在源自5日龄生殖嵴的基质细胞上进行培养。在7次实验中，4天内类胚体（MCs）数量平均增加了4.8倍。观察到5日龄胚胎生殖嵴中的内源性PGCs松散地附着于基质细胞，并且在4天的培养过程中它们也增加了3.8倍。这些结果表明将这种培养方法应用于转基因鸡生产的可能性。