Yokoyama A, Kohno N, Hirasawa Y, Kondo K, Abe M, Inoue Y, Fujioka S, Fujino S, Ishida S, Hiwada K
Second Department of Internal Medicine, Ehime University School of Medicine, Japan.
Clin Exp Immunol. 1995 May;100(2):325-9. doi: 10.1111/j.1365-2249.1995.tb03672.x.
We measured soluble IL-6 receptor (sIL-6R) levels in serum and bronchoalveolar lavage fluids (BALF) from patients with interstitial pneumonia of unknown etiology (IP) (n = 17), sarcoidosis (n = 8) and normal control subjects (n = 10), to investigate its role in pulmonary diseases. Soluble IL-6R was determined by an ELISA. The volume of epithelial lining fluid (ELF) in BALF was estimated using an urea method. We found that levels of sIL-6R in serum, BALF, and ELF from patients with IP or sarcoidosis were significantly higher than those from normal subjects. Furthermore, levels of sIL-6R in BALF or ELF were significantly correlated with those of albumin, indicating that sIL-6R, together with albumin, may enter ELF as a result of the increased permeability caused by pulmonary inflammation. Thus most of the sIL-6R in ELF would be from serum, and relatively small amounts of it might be produced locally. However, sIL-6R levels in ELF, but neither serum nor BALF, were significantly correlated with levels of C-reactive protein in patients with IP. These results suggest that both systemic and local production of sIL-6R are increased, and raised sIL-6R is involved in the modulation of systemic and local inflammatory responses in patients with IP and sarcoidosis.
我们检测了病因不明的间质性肺炎（IP）患者（n = 17）、结节病患者（n = 8）及正常对照者（n = 10）血清和支气管肺泡灌洗液（BALF）中可溶性白细胞介素-6受体（sIL-6R）水平，以研究其在肺部疾病中的作用。采用酶联免疫吸附测定法（ELISA）测定可溶性白细胞介素-6受体。使用尿素法估算BALF中上皮衬液（ELF）的体积。我们发现，IP患者或结节病患者血清、BALF和ELF中的sIL-6R水平显著高于正常受试者。此外，BALF或ELF中的sIL-6R水平与白蛋白水平显著相关，表明sIL-6R可能与白蛋白一起，因肺部炎症导致的通透性增加而进入ELF。因此，ELF中的大多数sIL-6R可能来自血清，而其局部产生的量可能相对较少。然而，在IP患者中，ELF中的sIL-6R水平与C反应蛋白水平显著相关，而血清和BALF中的sIL-6R水平与C反应蛋白水平无显著相关性。这些结果表明，IP和结节病患者中sIL-6R的全身和局部产生均增加，升高的sIL-6R参与调节全身和局部炎症反应。
