Desgeorges A, Gabay C, Silacci P, Novick D, Roux-Lombard P, Grau G, Dayer J M, Vischer T, Guerne P A
Division de Rhumatologie, Hôpital Universitaire, Geneva, Switzerland.
J Rheumatol. 1997 Aug;24(8):1510-6.
To determine levels of soluble interleukin 6 receptor-alpha (sIL-6R alpha) in synovial fluid (SF) and serum from patients with different rheumatic diseases, and to analyze its cellular origin compared to IL-6.
IL-6 and sIL-6R alpha concentrations were measured in sera, SF, and culture supernatants of different cells types using specific sandwich ELISA.
IL-6 levels were significantly higher (30 to 1000-fold) in SF than in sera, and higher in inflammatory arthropathies such as rheumatoid arthritis (RA), chondrocalcinosis, and gout than in osteoarthritis (OA). sIL-6R alpha levels in SF from patients with RA, gout, and chondrocalcinosis were also higher (24.7 +/- 7.5, 23.2 +/- 9.1, and 19.5 +/- 7.4 ng/ml, respectively) than in patients with OA (10.1 +/- 5 ng/ml), although the difference was distinctly smaller. In contrast, sIL-6R alpha concentrations did not differ significantly between the sera of healthy donors and patients. sIL-6R alpha levels were similar in SF and sera from inflammatory arthropathies, but lower in all osteoarthritic SF, compared to their corresponding serum. In contrast to IL-6, sIL-6R alpha was produced in high amounts by hepatocytes but not by structural cells of the joint (chondrocytes, synoviocytes, fibroblasts, and endothelial cells). Polymorphonuclear cells and mononuclear cells released intermediate levels. A significant correlation between sIL-6R alpha concentration and total number of leukocytes was observed in SF.
Elevated levels of sIL-6R alpha were found in serum, likely to result from a marked release by hepatocytes in vitro. That levels are higher in inflammatory SF may be due in part to release by inflammatory cells in situ.
测定不同风湿性疾病患者滑液（SF）和血清中可溶性白细胞介素6受体α（sIL-6Rα）的水平，并与白细胞介素6（IL-6）比较分析其细胞来源。
使用特异性夹心酶联免疫吸附测定法（ELISA）检测不同细胞类型的血清、SF和培养上清液中IL-6和sIL-6Rα的浓度。
SF中的IL-6水平显著高于血清（30至1000倍），在类风湿关节炎（RA）、软骨钙质沉着症和痛风等炎性关节病中高于骨关节炎（OA）。RA、痛风和软骨钙质沉着症患者SF中的sIL-6Rα水平（分别为24.7±7.5、23.2±9.1和19.5±7.4 ng/ml）也高于OA患者（10.1±5 ng/ml），尽管差异明显较小。相比之下，健康供体和患者血清中的sIL-6Rα浓度无显著差异。炎性关节病的SF和血清中sIL-6Rα水平相似，但所有骨关节炎SF中的sIL-6Rα水平均低于相应血清。与IL-6不同，sIL-6Rα由肝细胞大量产生，而不由关节结构细胞（软骨细胞、滑膜细胞、成纤维细胞和内皮细胞）产生。多形核细胞和单核细胞释放中等水平。在SF中观察到sIL-6Rα浓度与白细胞总数之间存在显著相关性。
血清中sIL-6Rα水平升高，可能是由于体外肝细胞的大量释放。炎性SF中水平较高可能部分归因于原位炎性细胞的释放。
