Marcon L, Fritz M E, Kurman C C, Jensen J C, Nelson D L
Immunophysiology Section, National Cancer Institute, Bethesda, MD 20892.
Clin Exp Immunol. 1988 Jul;73(1):29-33.
The T lymphocyte-derived lymphokine interleukin 2 and the cell-associated receptor for this molecule play major roles in the activation and regulation of the human immune response. An enzyme-linked immunosorbent assay has been developed to measure quantitatively a soluble form of one component of the human interleukin 2 receptor, namely the Tac peptide. In the present studies, soluble Tac peptide was measured in the urine of normal individuals (mean = 92 U/ml), a level not significantly different (0.01 less than P less than 0.05) from the corresponding serum concentrations (mean = 175). The urinary Tac peptide had a molecular weight of 40-45 kD by sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis and specifically bound interleukin 2. Elevated levels of urinary Tac peptide were found in four patients with adult T cell leukaemia who also had elevated serum levels of Tac peptide. Thus, urine may represent a valuable source of lymphokine-binding proteins that may serve as important markers of immunological activation.
T淋巴细胞衍生的淋巴因子白细胞介素2及其细胞相关受体在人类免疫反应的激活和调节中起主要作用。已开发出一种酶联免疫吸附测定法来定量检测人白细胞介素2受体一种成分的可溶性形式，即Tac肽。在本研究中，检测了正常个体尿液中的可溶性Tac肽（平均值 = 92 U/ml），该水平与相应血清浓度（平均值 = 175）无显著差异（0.01 ＜ P ＜ 0.05）。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析，尿Tac肽的分子量为40 - 45 kD，且能特异性结合白细胞介素2。在4例成人T细胞白血病患者中发现尿Tac肽水平升高，这些患者血清中Tac肽水平也升高。因此，尿液可能是淋巴因子结合蛋白的一个有价值的来源，这些蛋白可能是免疫激活的重要标志物。
