Liu Z, Meyerowitz E M
California Institute of Technology, Pasadena 91125, USA.
Development. 1995 Apr;121(4):975-91. doi: 10.1242/dev.121.4.975.
LEUNIG was identified in a genetic screen designed to isolate second-site enhancer mutations of the floral homeotic mutant apetala2-1. leunig mutations not only enhance apetala2, but by themselves cause a similar but less-pronounced homeotic transformation than apetala2 mutations. leunig flowers have sepals that are transformed toward stamens and carpels, and petals that are either staminoid or absent. In situ hybridization experiments with leunig mutants revealed altered expression pattern of the floral homeotic genes APETALA1, APETALA3, PISTILLATA, and AGAMOUS. Double mutants of leunig and agamous exhibited a phenotype similar to agamous single mutants, indicating that agamous is epistatic to leunig. Our analysis suggests that a key role of LEUNIG is to negatively regulate AGAMOUS expression in the first two whorls of the Arabidopsis flower.
LEUNIG是在一项旨在分离花同源异型突变体apetala2-1的第二位点增强子突变的遗传筛选中被鉴定出来的。leunig突变不仅增强了apetala2的表型，而且其自身会导致一种比apetala2突变更为相似但不太明显的同源异型转变。leunig突变体的花中，萼片会向雄蕊和心皮转变，花瓣或者呈雄蕊状，或者缺失。对leunig突变体进行的原位杂交实验表明，花同源异型基因APETALA1、APETALA3、PISTILLATA和AGAMOUS的表达模式发生了改变。leunig和agamous的双突变体表型与agamous单突变体相似，这表明agamous对leunig呈上位性。我们的分析表明，LEUNIG的一个关键作用是在拟南芥花的前两轮中负调控AGAMOUS的表达。
