过表达的ZF5基因产物是一种与GL1-Kruppel蛋白相关的c-myc结合蛋白，在小鼠细胞系中具有生长抑制活性。
Over-expressed ZF5 gene product, a c-myc-binding protein related to GL1-Kruppel protein, has a growth-suppressive activity in mouse cell lines.
作者信息
Numoto M, Yokoro K, Yanagihara K, Kamiya K, Niwa O
机构信息
Department of Molecular Pathology, Hiroshima University.
出版信息
Jpn J Cancer Res. 1995 Mar;86(3):277-83. doi: 10.1111/j.1349-7006.1995.tb03051.x.
Abstract
ZF5 encodes a zinc finger protein, which contains five C2H2-type zinc fingers showing homology with the zinc finger of the Kruppel family, and binds to two sites in the mouse c-myc promoter. We report the effect of over-expression of ZF5 on cell growth. ZnCl2 treatment suppressed the growth of a mouse fibroblast cell line (L cells) transfected with the wild-type ZF5 gene driven by the metallothionein promoter. Cells transfected with the wild-type ZF5 gene formed colonies two- to fivefold less efficiently than those transfected with the mutant ZF5 gene in P19, NIH3T3, 3T3-L1 and L cells. Over-expression of ZF5 did not cause c-myc down-regulation or arrest of the cell cycle, but increased the DNA content.
摘要
ZF5编码一种锌指蛋白，它含有五个C2H2型锌指，与Kruppel家族的锌指具有同源性，并与小鼠c-myc启动子中的两个位点结合。我们报告了ZF5过表达对细胞生长的影响。氯化锌处理抑制了用金属硫蛋白启动子驱动的野生型ZF5基因转染的小鼠成纤维细胞系（L细胞）的生长。在P19、NIH3T3、3T3-L1和L细胞中，转染野生型ZF5基因的细胞形成集落的效率比转染突变型ZF5基因的细胞低两到五倍。ZF5的过表达并未导致c-myc下调或细胞周期停滞，但增加了DNA含量。
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