Otsuka A J, Franco R, Yang B, Shim K H, Tang L Z, Zhang Y Y, Boontrakulpoontawee P, Jeyaprakash A, Hedgecock E, Wheaton V I
Department of Biological Sciences, Illinois State University, Normal 61790-4120, USA.
J Cell Biol. 1995 May;129(4):1081-92. doi: 10.1083/jcb.129.4.1081.
Caenorhabditis elegans unc-44 mutations result in aberrant axon guidance and fasciculation with inappropriate partners. The unc-44 gene was cloned by transposon tagging, and verified by genetic and molecular analyses of six transposon-induced alleles and their revertants. Nucleotide sequence analyses demonstrated that unc-44 encodes a series of putative ankyrin-related proteins, including AO49 ankyrin (1815 aa, 198.8 kD), AO66 ankyrin (1867 aa, 204 kD), and AO13 ankyrin (< or = 4700 aa, < or = 517 kD). In addition to the major set of approximately 6 kb alternatively spliced transcripts, minor transcripts were observed at approximately 3, 5, 7, and 14 kb. Evidence is provided that mutations in the approximately 14-kb AO13 ankyrin transcript are responsible for the neuronal defects. These molecular studies provide the first evidence that ankyrin-related molecules are required for axonal guidance.
秀丽隐杆线虫unc-44突变会导致轴突导向异常以及与不适当的伙伴发生成束。unc-44基因通过转座子标签法克隆，并通过对六个转座子诱导的等位基因及其回复体进行遗传和分子分析得到验证。核苷酸序列分析表明，unc-44编码一系列假定的锚蛋白相关蛋白，包括AO49锚蛋白（1815个氨基酸，198.8千道尔顿）、AO66锚蛋白（1867个氨基酸，204千道尔顿）和AO13锚蛋白（≤4700个氨基酸，≤517千道尔顿）。除了主要的约6kb可变剪接转录本外还观察到约3、5、7和14kb的次要转录本。有证据表明约14kb的AO13锚蛋白转录本中的突变是神经元缺陷的原因。这些分子研究首次证明了轴突导向需要锚蛋白相关分子。
