Sakamuro D, Furukawa T, Takegami T
Department of Microbiology, Kanazawa Medical University, Ishikawa, Japan.
J Virol. 1995 Jun;69(6):3893-6. doi: 10.1128/JVI.69.6.3893-3896.1995.
Clinical evidence suggests that hepatitis C virus (HCV) is etiologically involved in hepatic cancer and liver cirrhosis. To investigate whether the HCV nonstructural protein NS3 has oncogenic activity, NIH 3T3 cells were transfected with an expression vector containing cDNA for the 5'- or 3'-half sequence of the HCV genome segment encoding NS3. Only cells transfected with the 5'-half cDNA rapidly proliferated, lost contact inhibition, grew anchorage independently in soft agar, and formed tumors in nude mice. PCR analysis confirmed the presence of the 5'-half DNA in the transfectants. These results suggest that the 5' region of the HCV genome segment encoding NS3 is involved in cell transformation.
临床证据表明丙型肝炎病毒（HCV）在病因上与肝癌和肝硬化有关。为了研究HCV非结构蛋白NS3是否具有致癌活性，将含有编码NS3的HCV基因组片段5'-或3'-半序列cDNA的表达载体转染到NIH 3T3细胞中。只有用5'-半cDNA转染的细胞迅速增殖，失去接触抑制，在软琼脂中独立于锚定生长，并在裸鼠中形成肿瘤。PCR分析证实转染子中存在5'-半DNA。这些结果表明，编码NS3的HCV基因组片段的5'区域参与细胞转化。
