Kayser K, Kremer C, Tacke M
Department of Pathology, Thoraxklinik, Heidelberg, Germany.
In Vivo. 1993 Jul-Aug;7(4):387-91.
Histological sections of formalin-fixed, paraffin-embedded bronchial carcinomas comprising 241 surgical specimens (lobes and lungs) were Feulgen stained. The integrated optical density of the nuclei (IOD) and the minimum distance between their centers of gravity (DG) was measured with an automated image analyzing system consisting of a TV camera connected to a personal computer via a frame grapper. Intratumorous lymphocytes served as DNA standard (2C peak), and 300 nuclei were measured at minimum. The entropy of the IOD, the percentage of S-phases (PS) of tumor cells with an IOD > 3C and > 5C, and the 2CV mean standard deviation were measured. According the theory of thermodynamically open systems, the entropiefluss (current of entropy) was defined by the gradient IOD/time through the surface of the tumors which can be approximated by the formula IODPSDG/tumor volume. The following results were obtained: a) IOD of bronchial carcinoma is not associated with tumor size or lymph node infiltration (pT and pN stage). b) The minimum distance between tumor cells decreases with increasing tumor stage and increasing lymph node stage (pT, pN stage). c) The entropiefluss is low in limited and advanced tumor stages, and highest in tumors at moderately progressive tumor stages (pT2, pT3, pN1, pN2). Thus, in accordance with the theory of thermodynamically open systems, the entropiefluss reflects the "biological activity" of bronchial carcinoma.
对241个手术标本（肺叶和全肺）的福尔马林固定、石蜡包埋支气管癌组织切片进行福尔根染色。使用由通过图像采集卡连接到个人计算机的电视摄像机组成的自动图像分析系统，测量细胞核的积分光密度（IOD）及其重心之间的最小距离（DG）。瘤内淋巴细胞用作DNA标准（2C峰），至少测量300个细胞核。测量IOD的熵、IOD>3C和>5C的肿瘤细胞的S期百分比（PS）以及2CV平均标准差。根据热力学开放系统理论，熵流（熵流）由通过肿瘤表面的IOD/时间梯度定义，可近似用公式IODPSDG/肿瘤体积表示。得到以下结果：a）支气管癌的IOD与肿瘤大小或淋巴结浸润（pT和pN分期）无关。b）肿瘤细胞之间的最小距离随着肿瘤分期和淋巴结分期（pT、pN分期）的增加而减小。c）在局限性和进展期肿瘤阶段，熵流较低，在中度进展性肿瘤阶段（pT2、pT3、pN1、pN2）的肿瘤中熵流最高。因此，根据热力学开放系统理论，熵流反映了支气管癌的“生物学活性”。
