[三角叶薯蓣细胞悬浮培养物中甾体糖苷的组成与生物活性]

[Composition and biological activity of steroid glycosides from a suspended culture of Dioscorea deltoidea Wall cells].

作者信息

Vasil'eva I S, Paseshnichenko V A

出版信息

Prikl Biokhim Mikrobiol. 1995 Mar-Apr;31(2):238-42.

Abstract

Two major steroid spirostanol glycosides (oligospirostanosides): deltoside (melting point, 296 degrees C, [alpha]D10:-90.8 degrees C (c 5.0,Py) and dioscine (melting point, 285-285 degrees C, [alpha]D10:-106.5 degrees C (c 5.0, Py) and a minor glycoside diosgenin-3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside were isolated form cell suspensions of Discorea deltoidea after fermentation with beta-glucosidase. The cell suspensions contained these glycosides as the furostanol analogs (oligofurostanosides) deltoside, protodioscine, and delta 5-furosten-3 beta,22,26-triol-3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D- glucopyranoside. The native oligofurostanosides isolated from D. deltoidea cell suspensions have a high biological activity and can be used as phytobiostimulators in medicine, veterinary, and agriculture.

摘要

两种主要的甾体螺旋甾烷醇糖苷（寡螺旋甾烷糖苷）：三角叶薯蓣皂苷（熔点296℃，[α]D10：-90.8℃（c 5.0，吡啶））和薯蓣皂苷（熔点285 - 285℃，[α]D10：-106.5℃（c 5.0，吡啶））以及一种次要的糖苷薯蓣皂苷元-3-O-α-L-鼠李吡喃糖基-(1→2)-β-D-葡萄糖吡喃糖苷，是在用β-葡萄糖苷酶发酵三角叶薯蓣细胞悬浮液后分离得到的。细胞悬浮液中这些糖苷以呋甾烷醇类似物（寡呋甾烷糖苷）的形式存在，即三角叶薯蓣皂苷、原薯蓣皂苷和δ5-呋甾烯-3β,22,26-三醇-3-O-α-L-鼠李吡喃糖基-(1→2)-β-D-葡萄糖吡喃糖苷。从三角叶薯蓣细胞悬浮液中分离得到的天然寡呋甾烷糖苷具有很高的生物活性，可在医学、兽医学和农业中用作植物生物刺激剂。