Olczyk K
Department of Clinical Chemistry and Laboratory Diagnostics, Silesian Academy of Medicine, Sosnowiec, Poland.
Ann Biol Clin (Paris). 1994;52(10):711-6.
Significant quantitative differences between macromolecular components of the nuclei pulposi of prolapsed human intervertebral discs and those taken at necropsy were found. The nuclei pulposi of prolapsed discs contained about twice more collagen in comparison to control tissues. The amount of type I and type III collagens grew in a higher degree in comparison to type II of this protein. A distinctly higher solubility of the prolapsed discs collagen, together with an increased aldehyde content in solubilized collagen fractions were observed. A distinct decrease in the amount of glycosaminoglycans, especially CH-4-S and CH-6-S were found. Furthermore, a significant increase in elastin content and its susceptibility to the proteolytic action of elastase were found. The significance of these phenomena for pathobiochemistry of disc prolapse is discussed.
研究发现，脱出的人椎间盘髓核的大分子成分与尸检时获取的髓核在数量上存在显著差异。与对照组织相比，脱出椎间盘的髓核中胶原蛋白含量约多出两倍。与该蛋白的II型相比，I型和III型胶原蛋白的含量增长更为显著。观察到脱出椎间盘胶原蛋白的溶解度明显更高，同时溶解的胶原蛋白组分中的醛含量增加。发现糖胺聚糖的量明显减少，尤其是CH-4-S和CH-6-S。此外，还发现弹性蛋白含量显著增加及其对弹性蛋白酶蛋白水解作用的敏感性增强。本文讨论了这些现象对椎间盘脱出病理生物化学的意义。
