Olczyk K
Department of Clinical Chemistry and Laboratory Diagnostics, Silesian Academy of Medicine, Sosnowiec, Poland.
Z Rheumatol. 1994 Jan-Feb;53(1):19-25.
The age-related changes in glycosaminoglycan composition of human intervertebral discs were studied. A large number of tissue samples taken from 301 individuals at autopsy or at operation of prolapsed discs were investigated. It was found that the aging process is accompanied by a progressive decrease in GAGs content, both in annulus fibrosus and in nucleus pulposus. The GAG: collagen ratio significantly decreases. The KS and Ch-6-S were found to be the most abundant GAGs in both parts of the disc and their amounts decrease during the aging. A distinct, age-related increase of KS/Ch-S and Ch-6-S/Ch-4-S ratios was observed. Significant quantitative differences between the GAGs contents in normal and prolapsed discs were found. The nuclei pulposi of prolapsed discs contained less GAGs, especially KS and Ch-6-S, in comparison to normal tissue of the same age. Also, the age-related decline of GAGs: collagen ratio is more distinct in prolapsed discs. It seems that these differences in GAGs composition may be important in the mechanism of predisposition to disc prolapse.
对人类椎间盘糖胺聚糖组成的年龄相关性变化进行了研究。对从301例尸检或椎间盘突出症手术患者身上获取的大量组织样本进行了调查。结果发现，衰老过程伴随着纤维环和髓核中糖胺聚糖含量的逐渐下降。糖胺聚糖与胶原蛋白的比例显著降低。硫酸角质素（KS）和6-硫酸软骨素（Ch-6-S）被发现是椎间盘两部分中含量最丰富的糖胺聚糖，并且它们的含量在衰老过程中会减少。观察到KS/硫酸软骨素（Ch-S）和Ch-6-S/4-硫酸软骨素（Ch-4-S）比例存在明显的与年龄相关的增加。发现正常椎间盘和突出椎间盘的糖胺聚糖含量存在显著的定量差异。与同年龄的正常组织相比，突出椎间盘的髓核中糖胺聚糖含量较少，尤其是KS和Ch-6-S。此外，糖胺聚糖与胶原蛋白比例的年龄相关性下降在突出椎间盘中更为明显。这些糖胺聚糖组成的差异似乎在椎间盘突出易患机制中可能很重要。
