Manova K, De Leon V, Angeles M, Kalantry S, Giarré M, Attisano L, Wrana J, Bachvarova R F
Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021, USA.
Mech Dev. 1995 Jan;49(1-2):3-11. doi: 10.1016/0925-4773(94)00295-x.
Activin is a potent inducer of mesoderm in frog embryos. We showed previously that in the mouse, activin beta A is expressed in the uterine decidua near the embryo before and during the first appearance of mesoderm (E4.5-E6.5). Here, using Northern blotting and in situ hybridization, we show that mouse oocytes, E6.5 and E7.5 embryos, and E6.5 and E7.5 decidua contain mRNAs for both activin receptors type II and IIB. The expression of activin receptor type IIB is particularly strong in embryonic ectoderm apparent at E5.5 and continuing through E8.5. These results support the hypothesis that activin derived from the decidua promotes development of mesoderm in the period E5.5-E6.5.
激活素是青蛙胚胎中中胚层的一种强效诱导剂。我们之前表明，在小鼠中，激活素βA在中胚层首次出现之前及期间（E4.5 - E6.5），在靠近胚胎的子宫蜕膜中表达。在此，我们运用Northern印迹法和原位杂交技术，发现小鼠卵母细胞、E6.5和E7.5胚胎以及E6.5和E7.5蜕膜均含有II型和IIB型激活素受体的mRNA。IIB型激活素受体在E5.5时明显可见于胚胎外胚层，并持续至E8.5，其表达尤为强烈。这些结果支持了这样的假说，即来自蜕膜的激活素在E5.5 - E6.5期间促进中胚层的发育。
