Hill W G, Babiker H A, Ranford-Cartwright L C, Walliker D
Institute of Cell, Animal and Population Biology, University of Edinburgh.
Genet Res. 1995 Feb;65(1):53-61. doi: 10.1017/s0016672300033000.
Methods for estimating probability of identity by descent (f) are derived for data on numbers of genotypes at single loci and at pairs of loci with many alleles at each locus. The methods are general, but are specifically applied to data on genotype frequencies in zygotes of the malaria parasite sampled from its mosquito host in order to find the extent of outcrossing in the parasite and the degree of clonality in populations. It is assumed that zygotes are the outcome either of gametes of the same clone, in which they are identical at all loci, or are products of two random, unrelated clones. From the estimate of f an effective number of clones per human host can also be derived. For Plasmodium falciparum from a Tanzanian village, estimates of f are 0.33 from data on zygote frequencies at two multiallelic loci, indicating that two-thirds of zygotes produce recombinant type.
针对单个基因座以及每个基因座有多个等位基因的基因座对的基因型数量数据，推导了通过系谱同一性概率（f）进行估计的方法。这些方法具有通用性，但特别应用于从疟原虫的蚊子宿主中采样的疟原虫合子的基因型频率数据，以确定疟原虫的异交程度和群体中的克隆程度。假设合子要么是同一克隆的配子的产物，在这种情况下它们在所有基因座上都是相同的，要么是两个随机的、不相关克隆的产物。从f的估计值还可以推导出每个人类宿主的有效克隆数。对于来自坦桑尼亚一个村庄的恶性疟原虫，根据两个多等位基因座的合子频率数据，f的估计值为0.33，这表明三分之二的合子产生重组类型。
