Ellis J A, Hassard L E, Morley P S
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
J Am Vet Med Assoc. 1995 Feb 1;206(3):354-61.
A study was designed to determine if inactivated bovine respiratory syncytial virus (BRSV) vaccines induce the same types of antibody and cellular responses as does a modified-live BRSV vaccine. Ninety mixed-breed, 5- to 6-month-old beef calves were randomly assigned to 1 of 6 groups with 15 animals/group. Calves in 5 of the groups were inoculated on days 0 and 14 with 1 of 4 inactivated virus vaccines or with a modified-live virus vaccine. The remaining 15 calves were maintained as unvaccinated controls. Immune responses were measured on days 0 and 24, by means of ELISA, virus neutralization assay, blocking ELISA for the BRSV fusion (F) protein, immunoblotting, and lymphocyte blastogenesis assay. All vaccines induced production of antibodies that recognized the F protein; however, the ratio of neutralizing antibody titer to change in BRSV-specific IgG antibody concentration (as determined by use of ELISA) was lower for calves that received an inactivated virus vaccine than for calves that received the modified-live virus vaccine. All of the vaccines induced lymphocyte proliferative responses to BRSV. Results suggest that commercially employed inactivation processes can alter functionally important epitopes on BRSV envelope glycoproteins, leading to production of predominantly nonneutralizing antibodies in immunized cattle.
一项研究旨在确定灭活牛呼吸道合胞病毒（BRSV）疫苗是否能诱导与减毒活BRSV疫苗相同类型的抗体和细胞反应。90头5至6月龄的杂种肉牛犊被随机分为6组，每组15头。其中5组的牛犊在第0天和第14天接种4种灭活病毒疫苗之一或减毒活病毒疫苗。其余15头小牛作为未接种疫苗的对照。在第0天和第24天通过ELISA、病毒中和试验、针对BRSV融合（F）蛋白的阻断ELISA、免疫印迹和淋巴细胞增殖试验来测量免疫反应。所有疫苗均诱导产生了识别F蛋白的抗体；然而，接受灭活病毒疫苗的牛犊中和抗体滴度与BRSV特异性IgG抗体浓度变化（通过ELISA测定）的比值低于接受减毒活病毒疫苗的牛犊。所有疫苗均诱导了对BRSV的淋巴细胞增殖反应。结果表明，商业使用的灭活过程可能会改变BRSV包膜糖蛋白上功能重要的表位，导致免疫牛主要产生非中和抗体。
