Walravens K, Matheise J P, Knott I, Coppe P, Collard A, Didembourg C, Dessy F, Kettmann R, Letesson J J
Laboratoire de Microbiologie et d'Immunologie, Facultés Universitaires Notre-Dame de la Paix (F.U.N.D.P.), Namur, Belgium.
Arch Virol. 1996;141(12):2313-26. doi: 10.1007/BF01718633.
Bovine respiratory syncytial virus (BRSV) is a major cause of respiratory disease in calves. The BRSV genome encodes two major glycoproteins, G and F, which are the major targets for the host antibody response. We have expressed the F glycoprotein in insect cells (Sf9) using a recombinant baculovirus vector. A comparison of the F protein expressed in mammalian and insect cells by SDS-PAGE showed that only part of the baculovirus-produced protein was soluble and processed like the native protein. The antigenicity of the soluble form of the F protein expressed in insect cells was identical to that of the F protein expressed in mammalian cells. Immunization with the F protein expressed in insect cells induced neutralizing antibodies in mice. This antigenic preparation adjuvanted with Quil-A produced an increased neutralizing antibody titer and induced protection.
牛呼吸道合胞病毒（BRSV）是犊牛呼吸道疾病的主要病因。BRSV基因组编码两种主要糖蛋白G和F，它们是宿主抗体反应的主要靶点。我们使用重组杆状病毒载体在昆虫细胞（Sf9）中表达了F糖蛋白。通过SDS-PAGE对在哺乳动物细胞和昆虫细胞中表达的F蛋白进行比较，结果表明杆状病毒产生的蛋白只有一部分是可溶的，并且其加工方式与天然蛋白相同。在昆虫细胞中表达的F蛋白可溶形式的抗原性与在哺乳动物细胞中表达的F蛋白相同。用在昆虫细胞中表达的F蛋白免疫小鼠可诱导产生中和抗体。用Quil-A佐剂的这种抗原制剂产生了更高的中和抗体滴度并诱导了保护作用。
