使用捕获树脂灭活痰液中用于结核分枝杆菌DNA扩增的聚合酶抑制剂
Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin.
作者信息
Amicosante M, Richeldi L, Trenti G, Paone G, Campa M, Bisetti A, Saltini C
机构信息
Dipartimento di Scienze Mediche, Oncologiche e Radiologiche, Università di Modena, Italy.
出版信息
J Clin Microbiol. 1995 Mar;33(3):629-30. doi: 10.1128/jcm.33.3.629-630.1995.
Abstract
Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).
摘要
内源性PCR抑制剂会阻碍痰液样本中结核分枝杆菌DNA的扩增。使用阴离子结合树脂（GeneReleaser [GR]）可将PCR灵敏度从单独使用酚-氯仿提取法（P-C）时的77%（35个培养阳性样本中的27个）提高到91%（仅使用GR，不进行P-C；P>0.05）和100%（P-C与GR联合使用；P<0.05）。
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