Hashimoto Y, Itho Y, Fujinaga Y, Khan A Q, Sultana F, Miyake M, Hirose K, Yamamoto H, Ezaki T
Department of Microbiology, Gifu University School of Medicine, Japan.
J Clin Microbiol. 1995 Mar;33(3):775-7. doi: 10.1128/jcm.33.3.775-777.1995.
For a rapid diagnosis of typhoid fever, we developed a nested PCR based on the nucleotide sequence encoding the Vi antigen. All Salmonella typhi strains along with a Salmonella paratyphi C strain were PCR positive. This assay was able to detect S. typhi at the single-cell level.
为了快速诊断伤寒热，我们基于编码Vi抗原的核苷酸序列开发了一种巢式PCR。所有伤寒沙门氏菌菌株以及一株副伤寒沙门氏菌C菌株的PCR检测均呈阳性。该检测方法能够在单细胞水平检测到伤寒沙门氏菌。
