巢式聚合酶链反应在伤寒热诊断中的评估。
Evaluation of nested PCR in diagnosis of typhoid fever.
作者信息
Prakash Pradyot, Mishra Om Prakash, Singh Alok Kumar, Gulati Anil Kumar, Nath Gopal
机构信息
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
出版信息
J Clin Microbiol. 2005 Jan;43(1):431-2. doi: 10.1128/JCM.43.1.431-432.2005.
Abstract
In this study, nested PCR using H1-d primers, which is specific for Salmonella enterica serovar Typhi, was compared to blood culture and the single-tube Widal test. Results indicate that nested PCR can be used as a gold standard to determine the cutoff titer of the Widal test for diagnosis of typhoid fever.
摘要
在本研究中，使用针对伤寒沙门氏菌血清型伤寒杆菌的H1-d引物进行巢式PCR，并与血培养和单管肥达试验进行比较。结果表明，巢式PCR可作为确定肥达试验诊断伤寒热临界滴度的金标准。
相似文献
1
Evaluation of nested PCR in diagnosis of typhoid fever.巢式聚合酶链反应在伤寒热诊断中的评估。
J Clin Microbiol. 2005 Jan;43(1):431-2. doi: 10.1128/JCM.43.1.431-432.2005.
2
Detection of Salmonella typhi by nested polymerase chain reaction in blood, urine, and stool samples.通过巢式聚合酶链反应检测血液、尿液和粪便样本中的伤寒沙门氏菌。
Am J Trop Med Hyg. 2007 Jan;76(1):139-43.
3
Early Diagnosis of Typhoid By PCR For FliC-d Gene of Salmonella Typhi in Patients Taking Antibiotics.应用PCR技术检测服用抗生素患者伤寒杆菌鞭毛蛋白d基因（FliC-d）进行伤寒早期诊断
J Coll Physicians Surg Pak. 2015 Sep;25(9):662-6.
4
Diagnosis and Treatment of Typhoid Fever and Associated Prevailing Drug Resistance in Northern Ethiopia.埃塞俄比亚北部伤寒的诊断和治疗及相关流行耐药性。
Int J Infect Dis. 2015 Jun;35:96-102. doi: 10.1016/j.ijid.2015.04.014. Epub 2015 Apr 27.
5
Rapid diagnosis of typhoid fever by PCR assay using one pair of primers from flagellin gene of Salmonella typhi.使用一对来自伤寒沙门氏菌鞭毛蛋白基因的引物，通过聚合酶链反应（PCR）检测法快速诊断伤寒热。
J Infect Chemother. 2003 Sep;9(3):233-7. doi: 10.1007/s10156-003-0256-4.
6
Diagnosis of typhoid fever by polymerase chain reaction.通过聚合酶链反应诊断伤寒热。
Indian J Pediatr. 2007 Oct;74(10):909-13. doi: 10.1007/s12098-007-0167-y.
7
Evaluation of fliC-d based direct blood PCR assays for typhoid diagnosis.基于鞭毛蛋白C（fliC-d）的直接血液PCR检测法用于伤寒诊断的评估。
BMC Microbiol. 2016 Jun 13;16(1):108. doi: 10.1186/s12866-016-0723-6.
8
Multiplex PCR for differential diagnosis of emerging typhoidal pathogens directly from blood samples.直接从血液样本中进行多重PCR以鉴别新型伤寒病原体。
Epidemiol Infect. 2009 Jan;137(1):102-7. doi: 10.1017/S0950268808000654. Epub 2008 Apr 16.
9
A fast and highly sensitive blood culture PCR method for clinical detection of Salmonella enterica serovar Typhi.一种快速且高度敏感的血培养 PCR 方法，用于临床检测伤寒沙门氏菌血清型 Typhi。
Ann Clin Microbiol Antimicrob. 2010 Apr 19;9:14. doi: 10.1186/1476-0711-9-14.
10
Nested PCR for diagnosis of typhoid fever.用于伤寒热诊断的巢式聚合酶链反应
J Clin Microbiol. 2005 Sep;43(9):4920; author reply 4920. doi: 10.1128/JCM.43.9.4920.2005.
引用本文的文献
1
Novel Heptaplex PCR-Based Diagnostics for Enteric Fever Caused by Typhoidal Serovars and Its Applicability in Clinical Blood Culture.新型七重 PCR 检测方法在肠热症由伤寒血清型引起的诊断及其在临床血培养中的应用
J Microbiol Biotechnol. 2023 Nov 28;33(11):1457-1466. doi: 10.4014/jmb.2307.07031. Epub 2023 Aug 21.
2
From to Typhi: The Fascinating Journey of the Virulence and Pathogenicity of Typhi.从伤寒杆菌到伤寒沙门氏菌：伤寒杆菌毒力与致病性的迷人历程 。
需注意，原文中“From to Typhi”表述不太完整规范，推测可能前面和后面都缺失了具体的细菌名称等关键信息，这里暂且按常见的理解翻译，你可根据实际情况进一步确认和完善。
ACS Omega. 2023 Jul 14;8(29):25674-25697. doi: 10.1021/acsomega.3c02386. eCollection 2023 Jul 25.
3
Comparative accuracy of typhoid diagnostic tools: A Bayesian latent-class network analysis.伤寒诊断工具的比较准确性：贝叶斯潜在类别网络分析。
PLoS Negl Trop Dis. 2019 May 8;13(5):e0007303. doi: 10.1371/journal.pntd.0007303. eCollection 2019 May.
4
Persistent Infection and Long-Term Carriage of Typhoidal and Nontyphoidal Salmonellae.伤寒和非伤寒沙门氏菌的持续感染和长期携带。
Clin Microbiol Rev. 2018 Nov 28;32(1). doi: 10.1128/CMR.00088-18. Print 2019 Jan.
5
Evaluation of fliC-d based direct blood PCR assays for typhoid diagnosis.基于鞭毛蛋白C（fliC-d）的直接血液PCR检测法用于伤寒诊断的评估。
BMC Microbiol. 2016 Jun 13;16(1):108. doi: 10.1186/s12866-016-0723-6.
6
Development and Evaluation of a Blood Culture PCR Assay for Rapid Detection of Salmonella Paratyphi A in Clinical Samples.用于临床样本中甲型副伤寒沙门氏菌快速检测的血培养PCR检测方法的开发与评估
PLoS One. 2016 Mar 1;11(3):e0150576. doi: 10.1371/journal.pone.0150576. eCollection 2016.
7
Ulcerative Colitis and Its Association with Salmonella Species.溃疡性结肠炎及其与沙门氏菌属的关联。
Interdiscip Perspect Infect Dis. 2016;2016:5854285. doi: 10.1155/2016/5854285. Epub 2016 Jan 20.
8
A Meta-Analysis of Typhoid Diagnostic Accuracy Studies: A Recommendation to Adopt a Standardized Composite Reference.伤寒诊断准确性研究的荟萃分析：采用标准化综合参考标准的建议
PLoS One. 2015 Nov 13;10(11):e0142364. doi: 10.1371/journal.pone.0142364. eCollection 2015.
9
Epidemiology, Clinical Presentation, Laboratory Diagnosis, Antimicrobial Resistance, and Antimicrobial Management of Invasive Salmonella Infections.侵袭性沙门氏菌感染的流行病学、临床表现、实验室诊断、抗菌药物耐药性及抗菌药物管理
Clin Microbiol Rev. 2015 Oct;28(4):901-37. doi: 10.1128/CMR.00002-15.
10
Mix-infection of S. Typhi and ParaTyphi A in Typhoid Fever and Chronic Typhoid Carriers: A Nested PCR Based Study in North India.伤寒热和慢性伤寒带菌者中伤寒沙门氏菌和甲型副伤寒沙门氏菌的混合感染：印度北部一项基于巢式PCR的研究
J Clin Diagn Res. 2014 Nov;8(11):DC09-14. doi: 10.7860/JCDR/2014/9167.5107. Epub 2014 Nov 20.
本文引用的文献
1
Early detection of typhoid by polymerase chain reaction.
Ann Saudi Med. 1999 Jul-Aug;19(4):337-40. doi: 10.5144/0256-4947.1999.337.
2
Detection of Salmonella typhi by polymerase chain reaction: implications in diagnosis of typhoid fever.通过聚合酶链反应检测伤寒沙门氏菌：对伤寒热诊断的意义。
Infect Genet Evol. 2002 Dec;2(2):107-10. doi: 10.1016/s1567-1348(02)00090-4.
3
Widal agglutination test - 100 years later: still plagued by controversy.维达凝集试验——100年后：仍饱受争议。
Postgrad Med J. 2000 Feb;76(892):80-4. doi: 10.1136/pmj.76.892.80.
4
Quantitation of bacteria in blood of typhoid fever patients and relationship between counts and clinical features, transmissibility, and antibiotic resistance.伤寒热患者血液中细菌的定量分析以及菌数与临床特征、传播性和抗生素耐药性之间的关系。
J Clin Microbiol. 1998 Jun;36(6):1683-7. doi: 10.1128/JCM.36.6.1683-1687.1998.
5
Standardisation of polymerase chain reaction for the detection of Salmonella typhi in typhoid fever.用于检测伤寒热中伤寒沙门氏菌的聚合酶链反应标准化
J Clin Pathol. 1997 May;50(5):437-9. doi: 10.1136/jcp.50.5.437.
6
Interpretation of the Widal test in the diagnosis of typhoid fever in Bangladeshi children.孟加拉国儿童伤寒热诊断中肥达试验的解读
Ann Trop Paediatr. 1996 Mar;16(1):75-8. doi: 10.1080/02724936.1996.11747807.
7
Detection of Salmonella typhi in the blood of patients with typhoid fever by polymerase chain reaction.通过聚合酶链反应检测伤寒热患者血液中的伤寒沙门氏菌。
J Clin Microbiol. 1993 Jun;31(6):1439-43. doi: 10.1128/jcm.31.6.1439-1443.1993.
8
Detection of Salmonella typhi by PCR.通过聚合酶链反应检测伤寒沙门氏菌。
J Clin Microbiol. 1994 May;32(5):1415. doi: 10.1128/jcm.32.5.1415-.1994.
9
Development of nested PCR based on the ViaB sequence to detect Salmonella typhi.基于ViaB序列开发巢式PCR以检测伤寒沙门氏菌。
J Clin Microbiol. 1995 Mar;33(3):775-7. doi: 10.1128/jcm.33.3.775-777.1995.
10
The value of a single Widal test in the diagnosis of acute typhoid fever.单次肥达试验在急性伤寒热诊断中的价值。
Trop Geogr Med. 1981 Jun;33(2):113-6.
Zotero 插件