Yamashita Y, Hattori M, Oseto M, Mori M, Inouye H, Takagi K, Ishimaru Y, Nakano S
Ehime Prefectural Institute of Public Health.
Kansenshogaku Zasshi. 1995 Apr;69(4):377-82. doi: 10.11150/kansenshogakuzasshi1970.69.377.
Enteric adenoviruses (EAd), adenovirus (Ad) types 40 and 41, have been established as causative agents of gastroenteritis. By electron microscopic (EM) survey of acute gastroenteritis in children in the Matsuyama area, Ad were detected in 275 of 6476 fecal samples obtained from 1980 to 1993. Two-hundred-thirteen Ad-positive samples were tested for serotyping by the enzyme-linked immunosorbent assay (ELISA) using three monoclonal antibodies, Ad group-specific, Ad 40 type-specific and Ad41 type-specific antibody. Of 199 samples serotyped by ELISA, 65 were identified as Ad40, 73 as Ad41, 1 as double infection with Ad40 and Ad41, and 60 as Non-EAd. About 70% of Adenovirus detected by EM were suggested to be EAd. Other epidemiological feature was as follows: EAd were detected throughout the year. The predominant serotype was Ad40 during 1980-1985, while Ad41 were observed after 1986. EAd were detected most frequently from the children aged 0-3 years. The incidence of fever in EAd positive group was lower (30%) than that (67%) in the Non-EAd positive group. The incidence of vomiting, nausea and respiratory symptoms was higher in Ad41 associated infections than Ad40.
肠道腺病毒（EAd），即40型和41型腺病毒（Ad），已被确认为胃肠炎的病原体。通过对松山地区儿童急性胃肠炎进行电子显微镜（EM）检查，在1980年至1993年采集的6476份粪便样本中的275份中检测到了腺病毒。使用三种单克隆抗体，即腺病毒组特异性抗体、40型腺病毒特异性抗体和41型腺病毒特异性抗体，通过酶联免疫吸附测定（ELISA）对213份腺病毒阳性样本进行血清分型检测。在ELISA血清分型的199份样本中，65份被鉴定为40型腺病毒，73份为41型腺病毒，1份为40型和41型腺病毒双重感染，60份为非肠道腺病毒。通过电子显微镜检测到的腺病毒中约70%被认为是肠道腺病毒。其他流行病学特征如下：全年均可检测到肠道腺病毒。1980 - 1985年期间主要血清型为40型腺病毒，而1986年之后观察到的主要是41型腺病毒。肠道腺病毒在0 - 3岁儿童中检测最为频繁。肠道腺病毒阳性组的发热发生率（30%）低于非肠道腺病毒阳性组（67%）。41型腺病毒相关感染中呕吐、恶心和呼吸道症状的发生率高于40型腺病毒相关感染。
